Journal of Pharmacy And Bioallied Sciences
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SHORT COMMUNICATION
Year : 2010  |  Volume : 2  |  Issue : 4  |  Page : 369-371

Fluorescent labeling of human albumin using the new aromatic dialdehyde labels and the study of innerfilter effect


1 Institute of Pharmaceutical Sciences, Baqai Medical University, Toll Plaza, Super Highway, Gadap Road, Karachi - 746 00, Pakistan
2 Department of Chemistry, The Islamia University, Bahawalpur, Pakistan
3 Department of Analytical Chemistry, Loughborough University of Engineering & Technology, Loughborough, LE11, England

Correspondence Address:
Muhammd Ali Sheraz
Institute of Pharmaceutical Sciences, Baqai Medical University, Toll Plaza, Super Highway, Gadap Road, Karachi - 746 00
Pakistan
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Source of Support: None, Conflict of Interest: None


DOI: 10.4103/0975-7406.72143

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The labels naphthalene-2,3-dicarboxaldehyde (NDA), 1-phenylnaphthalene-2,3-dialdehyde (fNDA), and anthracene-2,3-dialdehyde (ADA) have been used as fluorigenic reagents. They formed fluorescent derivatives with proteins. The derivatives formed are in fact isoindoles. The fluorescence decay of the labels-antibody was found to extend over a period of 4, 8, and 10 h for ΦNDA, ADA, and NDA-derivative, respectively. Protein formed is comparatively less stable as compared to simple amino acids. In relation to innerfilter effect, the addition of cytochrome C, myoglobin, and ATP as absorbers to label-human albumin fluorophores appeared to have quenched the fluorescence. In the case of using NDA as label, the fluorescence was quenched roughly 70%, 24%, and 58% for addition of cytochrome C, myoglobin, and ATP, respectively. The labels used were found to give rapid, reproducible, and reliable results.


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