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 Table of Contents  
Year : 2012  |  Volume : 4  |  Issue : 1  |  Page : 10-20  

Current approaches toward production of secondary plant metabolites

1 Faculty of Pharmacy, Integral University, Lucknow, Uttar Pradesh, India
2 Department of Biotechnology, Integral, University, Lucknow, Uttar Pradesh, India

Date of Submission22-May-2011
Date of Decision23-Jun-2011
Date of Acceptance31-Jul-2011
Date of Web Publication9-Feb-2012

Correspondence Address:
Md. Sarfaraj Hussain
Faculty of Pharmacy, Integral University, Lucknow, Uttar Pradesh
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Source of Support: None, Conflict of Interest: None

DOI: 10.4103/0975-7406.92725

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Plants are the tremendous source for the discovery of new products with medicinal importance in drug development. Today several distinct chemicals derived from plants are important drugs, which are currently used in one or more countries in the world. Secondary metabolites are economically important as drugs, flavor and fragrances, dye and pigments, pesticides, and food additives. Many of the drugs sold today are simple synthetic modifications or copies of the naturally obtained substances. The evolving commercial importance of secondary metabolites has in recent years resulted in a great interest in secondary metabolism, particularly in the possibility of altering the production of bioactive plant metabolites by means of tissue culture technology. Plant cell and tissue culture technologies can be established routinely under sterile conditions from explants, such as plant leaves, stems, roots, and meristems for both the ways for multiplication and extraction of secondary metabolites. In vitro production of secondary metabolite in plant cell suspension cultures has been reported from various medicinal plants, and bioreactors are the key step for their commercial production. Based on this lime light, the present review is aimed to cover phytotherapeutic application and recent advancement for the production of some important plant pharmaceuticals.

Keywords: Cell suspension culture, medicinal plants, plant pharmaceuticals, secondary metabolites

How to cite this article:
Hussain M, Fareed S, Ansari S, Rahman M, Ahmad IZ, Mohd. Saeed. Current approaches toward production of secondary plant metabolites. J Pharm Bioall Sci 2012;4:10-20

How to cite this URL:
Hussain M, Fareed S, Ansari S, Rahman M, Ahmad IZ, Mohd. Saeed. Current approaches toward production of secondary plant metabolites. J Pharm Bioall Sci [serial online] 2012 [cited 2022 Dec 7];4:10-20. Available from:

Medicinal plants are the most exclusive source of life-saving drugs for majority of the world's population. The utilization of plant cells for the production of natural or recombinant compounds of commercial interest has gained increasing attention over past decades. [1] The secondary metabolites are known to play a major role in the adaptation of plants to their environment and also represent an important source of pharmaceuticals. [2] The metabolism is defined as the sum of all the biochemical reactions carried out by an organism. Primary metabolic pathways converge too few end products, while secondary metabolic pathways diverge too many products. Primary requires the cell to use nutrients in its surroundings such as low-molecular weight compounds for cellular activity. There are three potential pathways for primary metabolism: the Embden Meyerhof-Parnas Pathway (EMP), the Entner-Dourdorof pathway, and the hexose monophosphate (HMP) pathway. The EMP produces two molecules of pyruvate via triose phosphate intermediates. This pathway occurs most widely in animal, plant, fungal, yeast, and bacterial cells. Many microorganisms, however, use this pathway solely for glucose utilization. During primary metabolism, hexoses such as glucose are converted to single cell protein by yeasts and fungi. This is generally done by using a combination of EMP and HMP pathways, followed by the tricarboxylic acid (TCA) cycle and respiration. Plants produce a vast and diverse assortment of organic compounds, the great majority of which do not appear to participate directly in growth and development. These substances, traditionally referred to as secondary metabolites, often are differentially distributed among limited taxonomic groups within the plant kingdom. Their functions, many of which remain unknown, are being elucidated with increasing frequency. The primary metabolites, in contrast, such as phytosterols, acyl lipids, nucleotides, amino acids, and organic acids, are found in all plants and perform metabolic roles that are essential and usually evident. Although noted for the complexity of their chemical structures and biosynthetic pathways, natural products have been widely perceived as biologically insignificant and have historically received little attention from most plant biologists. Pharmaceutical organic chemists, however, have long been interested in these novel phytochemicals and have investigated their chemical properties extensively since the 1850s. Studies of natural products stimulated development of the separation techniques, spectroscopic approaches to structure elucidation, and synthetic methodologies that now constitute the foundation of contemporary organic chemistry. Interest in natural products was not purely academic but rather was prompted by their great utility as dyes, polymers, fibers, glues, oils, waxes, flavoring agents, perfumes, and drugs. Recognition of the biological properties of myriad natural products has fueled the current focus of this field, namely, the search for new drugs, antibiotics, insecticides, and herbicides. Based on their biosynthetic origins, plant natural products can be divided into three major groups: the terpenoids, the alkaloids, and the phenolic compounds. All terpenoids, including both primary metabolites and more than 25,000 secondary compounds, are derived from the five-carbon precursor isopentenyl diphosphate (IPP). The 12,000 or so known alkaloids, which contain one or more nitrogen atoms, are biosynthesized principally from amino acids. The 8000 or so phenolic compounds are formed by way of either the shikimic acid pathway or the malonate/acetate pathway. [3] In this review, we provide an overview of recent trends on production of secondary plant metabolites.

   Rationale of the Study Top

The objectives of this study were to get an overview of various works that have been done and could be done in the field of metabolic engineering of plant secondary metabolites by using yeast and to search the possibility of using methods and mechanisms for the production of various human health promoting plant secondary metabolites in the coming future. The principle advantage of recent technologies is that it may provide continuous, reliable source of plant pharmaceuticals and could be used for the large-scale culture of plant cells from which these metabolite can be extracted. Plant cell and tissue cultures hold great promise for controlled production of myriad of useful secondary metabolites on demand. The current yield and productivity cannot fulfill the commercial goal of plant cell-based bioprocess for the production of most secondary metabolites. In order to stretch the boundary, recent advances, new directions, and opportunities in plant cell-based processes are being critically examined. However, most often trials with plant cell cultures fail to produce the desired products. In such cases, strategies to improve the production of secondary metabolites must be considered. One of the main problems encountered is the lack of basic knowledge of the biosynthetic routes and mechanisms responsible for the production of plant metabolites. Where the productivity of the desired metabolites is limited by the lack of particular precursors, biotransformation using an exogenous supply of biosynthetic precursors, genetic manipulation, and metabolic engineering may improve the accumulation of compounds. Elicitors, compounds triggering the formation of secondary metabolites, can be abiotic or biotic. Natural elicitors include polysaccharides such as pectin and chitosan, which are also used in the immobilization and permeabilization of plant cells. Immobilization with suitable bioreactor system provides several advantages, such as continuous process operation, but for the development of an immobilized plant cell culture process, natural or artificially induced secretion of the accumulated product into the surrounding medium is necessary.

   Advancements in the Production of Secondary Metabolites Top

Plant cell and tissue cultures hold great promise for controlled production of myriad of useful secondary metabolites on demand. Discoveries of cell cultures capable of producing specific medicinal compounds at a rate similar or superior to that of intact plants have accelerated in the last few years has been summarized in [Table 1]. [4] In order to obtain high yields suitable for commercial exploitation, efforts have been focused on isolating the biosynthetic activities of cultured cells, achieved by optimizing the cultural conditions, selecting high-producing strains and employing precursor feeding, transformation methods, and immobilization techniques. [5] Transgenic hairy root cultures have revolutionized the role of plant tissue culture in secondary metabolite production. They are unique in their genetic and biosynthetic stability, faster in growth, and more easily maintained. Using this methodology, a wide range of chemical compounds has been synthesized. [6] Advances in tissue culture, combined with improvement in genetic engineering of pharmaceuticals, nutraceuticals, and other beneficial substances. [7] Recent advances in the molecular biology, enzymology, and fermentation technology of plant cell cultures suggest that these systems will become a viable source of important secondary metabolites. [8] Genome manipulation is resulting in relatively large amounts of desired compounds produced by plants infected with an engineered virus, whereas transgenic plants can maintain constant levels of production of proteins without additional intervention. [9] Large-scale plant tissue culture is found to be an attractive alternative approach to traditional methods of plantation as it offers controlled supply of biochemical's independent of plant availability. [10]
Table 1: Bioactive secondary metabolites from plant cell culture[4],[9],[61]

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   Production of Secondary Metabolites from Medicinal Plants by Plant Tissue Cultures Top

Due to these advances, research in the area of tissue culture technology for production of plant chemicals has bloomed beyond expectations. [4] The major advantages of a cell culture system over the conventional cultivation of whole plants are as follows:

  • Useful compounds can be produced under controlled conditions independent of climatic changes or soil conditions.
  • Cultured cells would be free of microbes and insects.
  • The cells of any plants, tropical or alpine, could easily be multiplied to yield their specific metabolites.
  • Automated control of cell growth and rational regulation of metabolite processes would reduce labor costs and improve productivity.
  • Organic substances are extractable from callus cultures.

   Trends in Production of Secondary Plant Metabolites from Higher Plants Top

Plant cell and tissue cultures can be established routinely under sterile conditions from explants, such as plant leaves, stems, roots, and meristems for multiplication and extraction of secondary metabolites. Strain improvement, methods for the selection of high-producing cell lines, and medium optimizations can lead to an enhancement in secondary metabolite production. The capacity for plant cell, tissue, and organ cultures to produce and accumulate many of the same valuable chemical compounds as the parent plant in nature has been recognized almost since the inception of in vitro technology. The strong and growing demand in today's marketplace for natural, renewable products has refocused attention on in vitro plant materials as potential factories for secondary phytochemical products and has paved the way for new research exploring secondary product expression in vitro. [11] There is a series of distinct advantages to producing a valuable secondary product in plant cell culture, rather than in vivo in the whole crop plant.

These include the following:

  • Production can be more reliable, simpler, and more predictable.
  • Isolation of the phytochemical can be rapid and efficient, when compared with extraction from complex whole plants.
  • Compounds produced in vitro can directly parallel compounds in the whole plant.
  • Interfering compounds that occur in the field-grown plant can be avoided in cell cultures.
  • Tissue and cell cultures can yield a source of defined standard phytochemicals in large volumes.
  • Tissue and cell cultures are a potential model to test elicitation.
  • Cell cultures can be radiolabeled, such that the accumulated secondary products, when provided as feed to laboratory animals, can be traced metabolically.
While research to date has succeeded in producing a wide range of valuable secondary phytochemicals in unorganized callus or suspension cultures, in other cases production requires more differentiated micro plant or organ cultures. [12] This situation often occurs when the metabolite of interest is only produced in specialized plant tissues or glands in the parent plant. A prime example is ginseng (Panax ginseng). Because saponin and other valuable metabolites are specifically produced in ginseng roots, root culture is required in vitro. Similarly, herbal plants such as Hypericum perforatum (St. John's wort), which accumulates the hypericins and hyperforins in foliar glands, have not demonstrated the ability to accumulate phytochemicals in undifferentiated cells. [13] As another example, biosynthesis of lysine to anabasine occurs in tobacco (Nicotiana tabacum) roots, followed by the conversion of anabasine to nicotine in leaves. Callus and shoot cultures of tobacco can produce only trace amounts of nicotine because they lack the organ-specific compound anabasine. In other cases, at least some degree of differentiation in a cell culture must occur before a product can be synthesized (e.g., vincristine or vinblastine from Catharanthus roseus). Reliance of a plant on a specialized structure for production of a secondary metabolite, in some cases, is a mechanism for keeping a potentially toxic compound sequestered. Intensive activities have been centered on production of natural drugs or chemoprotective compounds from plant cell culture by one or more of the following strategies:

   Organ Cultures for Secondary Metabolite Production Top

  • Fritillaria unibracteata can be rapidly propagated, directly from small cuttings of the bulb by the technique of organ culture. The cultured bulb can be harvested after a 50-day culture period in MS media supplemented with 4.44 - M BA and 5.71 - M IAA. The growth rate was about 30-50 times higher than that under natural wild growth conditions. The content of alkaloid and beneficial microelements in the cultured bulbs was higher than found in the wild bulb. [14]
  • In vitro shoot multiplication of Frangula alnus was obtained on woody plant medium with indole-3-acetic acid and 6-benzylaminapurine, the highest metabolite production (1731 mg/100 g of total anthraquinone was in the shoots grown on the MS medium with addition of 1-naphthilaceneacetic (NAA) (0.1 mg/l) and thidiazuron (TDZ) (0.1 mg/l). [15]

   Precursor Addition for Improvement of Secondary Metabolite Production Top

  • The treatment of plant cells with biotic and/or abiotic elicitors has been a useful strategy to enhance secondary metabolite production in cell cultures. [11] The most frequently used elicitors in previous studies were fungal carbohydrates, yeast extract, M,J and chitosan. MJ, a proven signal compound, is the most effective elicitor of taxol production in Taxus chinensis Roxb. [16] and gonsenoside production in P. ginseng C.A. Meyercell/organ culture. [17],[18],[19]
  • The involvement of amino acids in the biosynthesis of hyperforin and adhyperforin was reported in H. perforatum shoot cultures. Valine and isoleucine, upon administration to the shoot cultures, were incorporated into acyl side chain of hyperforin and adhyperforin, respectively. Feeding the shoot cultures with unlabelled lisoleucine at a concentration of 2 mM induced a 3-7-fold increase in the production of a hyperforin. [20] Production of triterpenes in leaf-derived callus and cell suspension cultures of Centella asiatica was enhanced by the feeding of amino acids. In the callus culture, manifold increase of asiaticoside accumulation was reported with the addition of leucine. [21]

   Elicitation of In vitro products Top

  • Plants and/or plant cells in vitro show physiological and morphological responses to microbial, physical, or chemical factors which are known as "elicitors." Elicitation is a process of inducing or enhancing synthesis of secondary metabolites by the plants to ensure their survival, persistence, and competitiveness. [11],[22] The study was applied in several abiotic elicitors to enhance growth and ginseng saponin biosynthesis in the hairy roots of P. ginseng. [23] Generally, elicitor treatments were found to inhibit the growth of the hairy roots, although simultaneously enhancing ginseng saponin biosynthesis. Tannic acid profoundly inhibited the hairy root growth during growth period.
  • The production of secondary metabolites in callus, cell suspension, and hairy roots of Ammi majus L. is by exposing them to elicitors: benzo (1, 2, 3)-thiadiazole-7-carbothionic acid S-methyl ester and autoclaved lysate of cell suspension of bacteria- Enterobacter sakazaki. [24] GC and GC-MS analysis of chloroform and methanol extracts indicated a higher accumulation of umbelliferone in the elicited tissues than in the control ones. Chitosan was the biotic elicitor polysaccharide and it is eliciting the manifold increase of anthraquinone production in Rubia akane cell culture. [25]

   Hairy Root Cultures as a Source of Secondary Metabolites Top

The hairy root system based on inoculation with Agrobacterium rhizogenes has become popular in the two last decades as a method of producing secondary metabolites synthesized in plant roots. [11],[26] The hairy root phenotype is characterized by fast hormone-independent growth, lack of geotropism, lateral branching, and genetic stability. The secondary metabolites produced by hairy roots arising from the infection of plant material by A. rhizogenes are the same as those usually synthesized in intact parent roots, with similar or higher yields. [27] This feature, together with genetic stability and generally rapid growth in simple media lacking phytohormones, makes them especially suitable for biochemical studies not easily undertaken with root cultures of an intact plant. During the infection process, A. rhizogenes transfers a part of the DNA (transferred DNA, T-DNA) located in the root-inducing plasmid Ri to plant cells, and the genes contained in this segment are expressed in the same way as the endogenous genes of the plant cells. Some A. rhizogenes, such as strain A4, have the T-DNA divided into two sections: the TR-DNA and TL-DNA, each of which can be incorporated separately into the plant genome. Two sets of pRi genes are involved in the root induction process: the aux genes located in the TR region of the pRi T-DNA and the rol (root loci) genes of the TL region. [28] The hairy roots are normally induced on aseptic, wounded parts of plants by inoculating them with A. rhizogenes.

   Genetic Manipulation in Hairy Root Culture for Secondary Metabolite Production Top

Transformed roots provide a promising alternative for the biotechnological exploitation of plant cells. A. rhizogenes-mediated transformation of plants may be used in a manner analogous to the well-known procedure employing Agrobacterium tumifaciens. A. rhizogenes-mediated transformation has also been used to produce transgenic hairy root cultures and plantlets have been regenerated. [11] None of the other T-DNA sequences are required for the transfer with the exception of the border sequences. The rest of the T-DNA can be replaced with the foreign DNA and introduced into cells from which whole plants can be regenerated. These foreign DNA sequences are stably inherited in a Mendelian manner. [29] The A. rhizogenes-mediated transformation has the advantage of being able to transfer any foreign gene of interest placed in binary vector to the transformed hairy root clone. An example of a gene of interest with regard to secondary metabolism that was introduced into hairy roots is the 6-hydroxylase gene of Hyoscyamus muticus which was introduced to hyocyamin-rich Atropa belladonna by a binary vector system using A. rhizogenes. [30] Engineered roots showed an increased amount of enzyme activity and a five-fold higher concentration of scopolamine.

   Role of Endophytes in In vitro Production of Secondary Metabolites Top

There are three schools of thought on the origins of secondary metabolism in plants. [11],[31] There is the argument that both plants and endophytic microbes coevolved with pathways to produce these natural products. Another thought is that an ancient horizontal gene transfer took place between plants and microbes. The third suggests that either plants or endophytic fungi produce these secondary metabolites and transfer them to the other symbiont. Biosynthetic pathway studies using radiolabeled precursor amino acids reveal that plants and endophytic fungi have similar but distinct metabolic pathways for production of secondary metabolites. [32] The question is whether bioactive phytochemicals of plants are produced by the plant itself or as a consequence of a mutualistic relationship with beneficial organisms in their tissue. The fact that a combination of inducing factors from both plants and endophytic fungi increased the accumulation of secondary metabolites in plants and fungi, respectively, [33],[34] suggest that the fungal endophyte may play important roles in the biosynthesis of secondary metabolites. Therefore, the symbiotic association and effects of plants and endophytes on each other during the production of other important pharmacological bioactive natural products such as camptothecin, vinblastine, and podophyllotoxin need to be explored. This could provide the framework for future natural product production through genetic and metabolic engineering. [35]

   Bioreactors Scaling up of Production of Secondary Metabolites Top

This is the application of bioreactor system for large-scale cultivation of plant cells for the production of valuable bioactive compounds in an active field. Plant cells in liquid suspension offer a unique combination of physical and chemical environments that must be accommodated in large-scale bioreactor process.

   Immobilization Scaling up of Secondary Metabolite Accumulation Top

  • Advances in scale-up approaches and immobilization techniques contribute to a considerable increase in the number of applications of plant cell cultures for the production of compounds with a high added value. Plant-derived compounds with cancer chemotherapeutic or antioxidant properties use rosmarinic acid and taxol as representative examples.
  • Cell cultures of Plumbago rosea were immobilized in calcium alginate and cultured in Murashige and Skoog's basal medium containing 10 mM CaCl 2 for the production of plumbagin, an important medicinal compound. [36] Studies were carried to find out the impact of immobilization on the increased accumulation of this secondary metabolite. Immobilization in calcium alginate enhanced the production of plumbagin by three-, two-, and one-folds compared with that of control, un-crosslinked alginate and CaCl 2 -treated cells, respectively.

   Tissue Cultures Producing Pharmaceutical Products of Interest Top

Research in the area of plant tissue culture technology has resulted in the production of many pharmaceutical substances for new therapeutics. Advances in the area of cell cultures for the production of medicinal compounds have made possible the production of a wide variety of pharmaceuticals such as alkaloids, terpenoids, steroids, saponins, phenolics, flavanoids, and amino acids. Successful attempts to produce some of these valuable pharmaceuticals in relatively large quantities by cell cultures are illustrated. [37],[9]


Taxol (paclitaxel), a complex diterpene alkaloid found in the bark of the Taxus tree, is one of the most promising anticancer agents known due to its unique mode of action on the microtubular cell system [Figure 1]. [38] At present, production of taxol by various Taxus species cells in cultures has been one of the most extensively explored areas of plant cell cultures in recent years owing to the enormous commercial value of taxol, the scarcity of the Taxus tree, and the costly synthetic process. [39],[40] In order to increase the taxoid production in these cultures, the addition of different amino acids to the culture medium was studied, and phenylalanine was found to assist in maximum taxol production in Taxus cuspidata cultures. [41] The influence of biotic and abiotic elicitors was also studied to improve the production and accumulation of taxol through tissue cultures. [42],[43],[ 44]
Figure 1: Chemical structure of taxol

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Morphine and codeine

Latex from the opium poppy, Papaver somniferum, is a commercial source of the analgesics, morphine, and codeine. Callus and suspension cultures of P. somniferum are being investigated as an alternative means for the production of these compounds [Figure 2]. Production of morphine and codeine in morphologically undifferentiated cultures has been reported. [45],[46] Without exogenous hormones, maximum codeine and morphine concentrations were 3.0 mg/g dry weight and 2.5 mg/g dry weight, respectively, up to three times higher than in cultures supplied with hormones. Biotransformation of codeinone to codeine with immobilized cells of P. somniferum has been reported by Furuya et al. (1972). [47] The conversion yield was 70.4%, and about 88% of the codeine converted was excreted into the medium.
Figure 2: Chemical structure of morphine and codeine

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L-3, 4-dihydroxyphenylalanine, is an important intermediate of secondary metabolism in higher plants and is known as a precursor of alkaloids, betalain, and melanine, isolated from Vinca faba, [48] Mucuna, Baptisia, and Lupinus. [49] It is also a precursor of catecholamines in animals and is being used as a potent drug for Parkinson's disease, a progressive disabling disorder associated with a deficiency of dopamine in the brain [Figure 3]. The widespread application of this therapy created a demand for large quantities of L-DOPA at an economical price level and this led to the introduction of cell cultures as an alternative means for enriched production. Brain [49] found that the callus tissue of Mucuna pruriense accumulated 25 mg/l DOPA in the medium containing relatively high concentrations of 2, 4-D. Teramoto and Komamine (1988) induced callus tissues of Mucuna hassjoo, M. Pruriense, and Mucuna deeringiana and optimized the culture conditions. The highest concentration of DOPA was obtained when M. hassjoo cells were cultivated in MS medium with 0.025 mg/l 2.4-D and 10 mg/l kinetin.
Figure 3: Chemical structure of 3-(3′, 4′-dihydroxyphenyl)-L-alanine (L-DOPA)

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Diosgenin is a precursor for the chemical synthesis of steroidal drugs and is tremendously important to the pharmaceutical industry. [50] In 1983, Tal et al. [51] reported on the use of cell cultures of Dioscorea deltoidea for the production of diosgenin [Figure 4]. They found that carbon and nitrogen levels greatly influenced diosgenin accumulation in one cell line. Ishida (1988) established Dioscorea immobilized cell cultures, in which reticulated polyurethane foam was shown to stimulate diosgenin production, increasing the cellular concentration by 40% and total yield by 25%. Tal et al. [50] have been able to obtain diosgenin levels as high as 8% in batch-grown D. deltoidea cell suspensions.
Figure 4: Chemical structure of diosgenin

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Capsaicin, an alkaloid, is used mainly as a pungent food additive in formulated foods. [52] It is obtained from fruits of green pepper (Capsicum spp.). Capsaicin is also used in pharmaceutical preparations as a digestive stimulant and for rheumatic disorders. [53] Suspension cultures of Capsicum frutescens produce low levels of capsaicin, but immobilizing the cells in reticulated polyurethane foam can increase production approximately 100-fold. Further improvements in productivity can be brought about by supplying precursors such as isocapric acid. Lindsey [54] reported that treatments, which suppress cell growth and primary metabolism, seem to improve capsaicin synthesis. A biotechnological process has been developed for the production of capsaicin from C. frutescens cells. Holden et al. [55] have reported elicitation of capsaicin in cell cultures of C. frutescens by spores of Gliccladium deliquescens. The effects of nutritional stress on capsaicin production in immobilized cell cultures of Capsicum annum were studied thoroughly by Ravishankar and Ramachandra Rao. [56] Biotransformation of externally fed protocatechuic aldehyde and caffeic acid to capsaicin in freely suspended cells and immobilized cells cultures of Capsicum frutescens has also been reported. [57]


Campothecin, a potent antitumor alkaloid, was isolated from Camptotheca acuminata. [58] Sakato and Misawa [59] induced C. acuminata callus on MS medium containing 0.2 mg/l 2,4-D and l mg/l kinetin and developed liquid cultures in the presence of gibberellin, l-tryptophan, and conditioned medium, which yielded camptothecin at about 0.0025% on a dry weight basis. When the cultures were grown on MS medium containing 4 mg/l NAA, accumulation of camptothecin reached 0.998 mg/l. [60]


Berberine is an isoquinoline alkaloid found in the roots of Coptis japonica and cortex of Phellondendron amurense. This antibacterial alkaloid has been identified from a number of cell cultures, notably those of C. japonica, [61],[62],[63] Thalictrum spp., [64],[65] and Berberis spp. [62] The productivity of berberine was increased in cell cultures by optimizing the nutrients in the growth medium and the levels of phytohormones. [63],[66],[67] By selecting high-yielding cell lines, Mitsui group produced berberine on a large scale with a productivity of 1.4 g/l over 2 weeks. Other methods for increasing yields include elicitation of cultures with a yeast polysaccharide elicitor, which has been successful with a relatively low-producing Thalictrum rugosum culture. [68] The influence of spermidine on berberine production in Thalictrum minus cell cultures has been reported by Hara et al. [69]

   Metabolic Engineering and Production of Secondary Metabolites Top

Metabolic engineering involves the targeted and purposeful alteration of metabolic pathways found in an organism to achieve better understanding and use of cellular pathways for chemical transformation, energy transduction, and supramolecular assembly. [70] This technique applied to plants will permit endogenous biochemical pathways to be manipulated and results in the generation of transgenic crops in which the range, scope, or nature of a plant's existing natural products are modified to provide beneficial commercial, agronomic, and/or postharvest processing characteristics. [71]

  • As in many cases production is too low for commercialization, metabolic engineering can provide various strategies to:
  • Improve productivity, such as increasing the number of producing cells.
  • Increasing the carbon flux through a biosynthetic pathway by overexpression of genes.
  • Codify for rate-limiting enzymes or blocking the mechanism of feedback inhibition and competitive pathways.
  • Decrease catabolism.
Several genes in the biosynthetic pathways for scopolamine, nicotine, and berberine have been cloned, making the metabolic engineering of these alkaloids possible. Expression of two branching-point enzymes was engineered: putrescine N-methyltransferase (PMT) in transgenic plants of Atropa belladonna and Nicotiana sylvestris and (S)-scoulerine 9-O-methyltransferase (SMT) in cultured cells of C. japonica and Eschscholzia californica. Overexpression of PMT increased the nicotine content in N. sylvestris, whereas suppression of endogenous PMT activity severely decreased the nicotine content and induced abnormal morphologies. Ectopic expression of SMT caused the accumulation of benzylisoquinoline alkaloids in E. californica. [72]

   Metabolic Engineering of Yeast for the Production of Plant Secondary Metabolites Top

Metabolic engineering is the alteration of cellular activities by the manipulation of enzymatic, transport, and regulatory functions of the cell by using recombinant DNA technology. Difficulty to obtain sufficient amounts of desired plant, slow growth of plants, varying composition and concentration depending on the geographical position and climatic conditions, and low yield of isolated compounds are some of the limitations of commercial extraction of these compounds by using plant as a single resource. On the other hand, some of the bottlenecks of chemical synthesis may include higher energy requirements, pollution, low reaction load due to unwanted chemical reactions, cost and availability of starting materials, and cost of separating and purifying the end products. For the metabolic engineering of plant secondary metabolites, it is necessary to know the biosynthetic pathways of those compounds, rate-limiting steps, and enzymes involved [Figure 5] and [Figure 6].
Figure 5: The acetate/mevalonate pathway for the formation of IPP, the basic five-carbon unit of terpenoid biosynthesis. Synthesis of each IPP unit requires three molecules of acetyl-CoA

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Figure 6: The major subclasses of terpenoids are biosynthesized from the basic five-carbon unit, IPP, and from the initial prenyl (allylic) diphosphate, dimethylallyl diphosphate, which is formed by isomerization of IPP. In reactions catalyzed by prenyltransferases, monoterpenes (C10), sesquiterpenes (C15), and diterpenes (C20) are derived from the corresponding intermediates by sequential head-to-tail addition of C5 units. Triterpenes (C30) are formed from two C15 (farnesyl) units joined head-to-head, and tetraterpenes (C40) are formed from two C20 (geranylgeranyl) units joined head-to-head

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  1. Production of flavonoids in yeast
  2. Production of terpenoids in yeast
    1. Production of monoterpenoids in yeast
    2. Production of sesquiterpenes in yeast
    3. Production of carotenoids in yeast
  3. Production of plant-origin alkaloids in yeast

   Production of Flavonoids in Yeast Top

Flavonoids are produced in yeast by expressing phenylpropanoid pathway. Many flavonoid compounds are successfully produced in yeast by cloning genes from different plant species and microorganisms. Flavanone has been successfully produced in yeast by expressing phenyl ammonia lyase (PAL), cinnamate-4-hydroxylase (C4H), 4-coumarate-CoA (4CL), and chalcone synthase (CHS) genes. [73] Flavones have also been produced in flavanone-producing recombinant yeast by expressing flavone synthase I (FSI) and flavone synthase II (FSII) genes.

Production of terpenoids in yeast

The biosynthesis of terpenes in higher plant cells shows two entirely separate enzymatic pathways: mevalonic acid pathway (MVA) and methylerythritol 4-phosphate pathway [Figure 5]. In yeast, only MVA pathway is involved in the biosynthesis of ergosterol as the major end product.

Production of monoterpenoids in yeast

Oswald et al.[74] engineered yeasts to produce monoterpenoids by expressing linalool synthase and geraniol synthase genes, and yeast strains successfully produced those monoterpenoid alcohols by using internal geranyl pyrophosphate.

Production of sesquiterpenes in yeast

Sesquiterpenes are the most diverse class of isoprenoids; some of them are interesting and extremely important compounds in human health because of their potent anticancer, antitumor, cytotoxic, antiviral, and antibiotic properties. Amorphadiene, a sesquiterpene of the antimalarial drug artemisin, is synthesized by the cyclization of farnesyl pyrophosphate (FPP). Ro et al.[75] used Saccharomyces cerevisiae to produce high amount of artemisinic acid using an engineered mevalonate pathway, amorphadiene synthase, and a novel cytochrome P450 monooxygenase from Artemisia annua.

Production of carotenoids in yeast

Schizosaccharomyces pombe
, a noncarotenogenic yeast, is not able to produce any carotenoids but it synthesizes ergosterol from FPP through the sterol biosynthetic pathway. Gunel et al.[76] cloned a gene encoding geranyl geranyl pyrophosphate synthase from bell pepper (C. annum) in S. pombe and successfully redirected carbon flow from the terpenoid pathway leading to ergosterol formation toward the production of carotenoid through the heterologous expression of carotenoid biosynthetic gene in a noncarotenogenic yeast, S. pombe.

Production of plant-origin alkaloids in yeast

Geerlings et al.[77] expressed genes coding for strictosidine synthase and strictosidine glucosidase enzymes from medicinal plant C. roseus in S. cerevisiae and successfully produced cathenamine from tryptamine and secologanin by functionally expressing those two enzymes in yeast. Along with the increased knowledge on biosynthetic pathways of many plant secondary metabolites, utilities of different yeast species should be investigated in future for the efficient microbial production of such compounds. Overcoming rate-limiting steps, reducing flux through competitive pathways, reducing catabolism, and over expression of regulatory genes are some of the strategies that can be used for increased secondary metabolites production through metabolic engineering.

   Conclusion Top

In future, metabolic engineering and biotechnological approaches can be used as an alternative production system to overcome the limited availability of biologically active, commercially valuable, and medicinally important plant secondary metabolite compounds. Advances in biotechniques, particularly methods for culturing plant cell cultures, should provide new means for the commercial processing of even rare plants and the chemicals they provide. The advantage of this method is that it can ultimately provide a continuous, reliable source of natural products. The major advantage of the cell cultures includes synthesis of bioactive secondary metabolites, running in controlled environment, independently from climate and soil conditions. The use of in vitro plant cell culture for the production of chemicals and pharmaceuticals has made great strides building on advances in plant science. The increased use of genetic tools and an emerging picture of the structure and regulation of pathways for secondary metabolism will provide the basis for the production of commercially acceptable levels of product. Knowledge of biosynthetic pathways of desired phytochemicals in plants and in cultures is often still in its infancy, and consequently strategies needed to develop an information based on a cellular and molecular level. These results show that in vitro plant cell cultures have potential for commercial production of secondary metabolites. The introduction of newer techniques of molecular biology, so as to produce transgenic cultures and to effect the expression and regulation of biosynthetic pathways, is also likely to be a significant step toward making cell cultures more generally applicable to the commercial production of secondary metabolites. The commercial values of plant secondary metabolites have been the main impetus for the enormous research effort put into understanding and manipulating their biosynthesis using various chemical, physiological, and biotechnological pathways. In vitro propagation of medicinal plants with enriched bioactive principles and cell culture methodologies for selective metabolite production is found to be highly useful for commercial production of medicinally important compounds. The increased use of plant cell culture systems in recent years is perhaps due to an improved understanding of the secondary metabolite pathway in economically important plants. Advances in plant cell cultures could provide new means for the cost-effective, commercial production of even rare or exotic plants, their cells, and the chemicals that they will produce. Knowledge of the biosynthetic pathways of desired compounds in plants as well as of cultures is often still rudimentary, and strategies are consequently needed to develop information based on a cellular and molecular level. A key to the evaluation of strategies to improve productivity is the realization that all the problems must be seen in a holistic context. At any rate, substantial progress in improving secondary metabolite production from plant cell cultures has been made within last few years. These new technologies will serve to extend and enhance the continued usefulness of higher plants as renewable sources of chemicals, especially medicinal compounds. We hope that a continuation and intensification efforts in this field will lead to controllable and successful biotechnological production of specific, valuable, and as yet unknown plant chemicals.

   Acknowledgments Top

We are grateful to Dr. K.F.H. Nazeer Ahamed, Assistant Professor, Department of Pharmacology, Vel's College of Pharmacy, Chennai, for his assistance and encouragement. We extend our sincere thanks to Mr. Md. Zaheen Hassan Ansari, research scholar, Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Jamia Hamdard, New Delhi, for critically reading the manuscript and providing the valuable suggestions. [102]

   References Top

1.Canter PH, Thomas H, Ernst E. Bringing medicinal plants into cultivation: opportunities and challenges for biotechnology. Trends Biotechnol 2005;23:180-5.  Back to cited text no. 1
2.Rao SR, Ravishankar GA. Plant cell cultures: Chemical factories of secondary metabolites. Biotechnol Adv 2002; 20:101-53.  Back to cited text no. 2
3.Rodney C, Toni M, Kutchan N, Lewis G. Natural Products. Biochemistry and molecular biology of plants. Buchanan B, Gruissem W, Jones R. editors; 2000. p. 1253-348.  Back to cited text no. 3
4.Vijaya SN, Udayasri PV, Aswani KY, Ravi BB, Phani KY, Vijay VM. Advancements in the production of secondary metabolites. J Nat Prod 2010;3:112-23.  Back to cited text no. 4
5.DiCosmo F, Misawa M. Plant cell and tissue culture: alternatives for metabolite production. Biotechnol Adv 1995;13:425-53.  Back to cited text no. 5
6.Giri A, Narasu ML. Transgenic hairy roots. recent trends and applications. Biotechnol Adv 2000;18:1-22.  Back to cited text no. 6
7.Hansen G, Wright MS. Recent advances in the transformation of plants. Trends Plant Sci 1999;4:226-31.  Back to cited text no. 7
8.Abdin MZ. Enhancing bioactive molecules in medicinal plants. In: Y Zhu, B Tan, B Bay, C Liu, editors. Natural Products-Essential resources for human. Singapore: World Scientific Publishing Co. Pvt. Ltd; 2007. p. 45-57.  Back to cited text no. 8
9.Abdin MZ, Kamaluddin A. Improving quality of medicinal herbs through physico-chemical and molecular approaches. In: MZ Abdin, YP Abrol, Narosa, editors. Traditional systems of medicine. India: Publishing House Pvt. Ltd; 2006. p. 30-9.  Back to cited text no. 9
10.Sajc LD, Grubisic G, Vunjak- Novakovic. Bioreactors for plant engineering: an outlook for further research. Biochem Eng J 2000; 4:89-99.  Back to cited text no. 10
11.Karuppusamy S. A review on trends in production of secondary metabolites from higher plants by in vitro tissue, organ and cell cultures. J Med Plants Res 2009;3:1222-39.  Back to cited text no. 11
12.Davioud E, Kan C, Hamon J, Tempe J, Husson HP. Production of indole alkaloids by in vitro root cultures from Catharanthus trichophyllus. Phytochemistry 1989;28:2675-80.  Back to cited text no. 12
13.Smetanska I. Production of secondary metabolites using plant cell cultures. Adv Biochem Eng Biotechnol 2008;111:187-228.  Back to cited text no. 13
14.Gao SL, Zhu DN, Cai ZH, Jiang Y, Xu DR. Organ culture of a precious Chinese medicinal plant - Fritillaria unibracteata. Plant Cell Tiss Org Cult 2004;59:197-201.  Back to cited text no. 14
15.Namdeo AG. Plant cell elicitation for production of secondary metabolites: A review. Pharmacog Rev 2007;1:69-79.  Back to cited text no. 15
16.Wink M, Alfermann AW, Franke R, Wetterauer B, Distl M, Windhovel J, et al. Sustainable bioproduction of phytochemicals by plant in vitro cultures: anticancer agents. Plant Genetic Resour 2008;12:113-23.  Back to cited text no. 16
17.Xu H, Kim YK, Suh SY, Udin MR, Lee SY, Park SU. Deoursin production from hairy root culture of Angelica gigas. J Korea Soc Appl Biol Chem 2008;51:349-51.  Back to cited text no. 17
18.Yagi A, Shoyama Y, Nishioka I. Formation of tetrahydroanthrcence glucosides by callus tissue of Aloe saponaria. Phytochemistry 1983; 22:1483-4.  Back to cited text no. 18
19.Yamanaka M, Ishibhasi K, Shimomura K, Ishimaru K. Polyacetylene glucosides in hairy root cultures of Lobelia cardinalis. Phytochemistry 1996;41:183-5.  Back to cited text no. 19
20.Kim OT, Kim MY, Hong MH, Ahn JC, Hwang B. Stimulation of asiticoside accumulation in the whole plant cultures of Centella asiatica (L.) urban by elicitors. Plant Cell Rep 2004;23:339-44.  Back to cited text no. 20
21.Karppinen K, Hokkanen J, Tolonen A, Mattila S, Hohtola A. Biosynthesis of hyperforin and adhyperforin from amino acid precursors in shoot cultures of Hypericum perforatum. Phytochemistry 2007;68:1038-45.  Back to cited text no. 21
22.Kiong AL, Mahmood M, Fodzillan NM, Daud SK. Effects of precursor supplementation on the production of triterpenes by Centella asiatica callus culture. Pak J Biol Sci 2005;8:1160-9.  Back to cited text no. 22
23.Jeong GA, Park DH. Enhanced secondary metabolite biosynthesis by elicitation in transformed plant root system: effect of abiotic elicitors. Appl Biochem Biotechnol 2006;129:436-46.  Back to cited text no. 23
24.Staniszewska I, Krolicka A, Mali E, Ojkowska E, Szafranek J. Elicitation of secondary metabolites in in vitro cultures of Ammi majus L. Enzyme Microbiol Technol 2003; 33:565-8.  Back to cited text no. 24
25.Jin JH, Shin JH, Kim JH, Chung IS, Lee HJ. Effect of chitosan elicitation and media components on the production of anthraquinone colorants in madder (Rubia akane Nakai) cell culture. Biotechnol Bioprocess Eng 1999;4:300-4.  Back to cited text no. 25
26.Palazon J, Pinol MT, Cusido RM, Morales C, Bonfill M. Application of transformed root technology to the production of bioactive metabolites. Recent Res Dev Plant Phys 1997;1:125-43.  Back to cited text no. 26
27.Sevón N, Oksman-Caldentey KM. Agrobacterium rhizogenes-mediated transformation: root cultures as a source of alkaloids. Planta Med 2002;68:859-68.  Back to cited text no. 27
28.Jouanin L. Restriction map of an agropine-type Ri plasmid and its homologies with Ti plasmids. Plasmid 1984;12:91-102.  Back to cited text no. 28
29.Zambryski P, Tempe J, Schell J. Transfer and function of T-DNA genes from Agrobacterium Ti and Ri plasmids in plants. Cell 1989;56:193-201.  Back to cited text no. 29
30.Hashimoto T, Yun DJ, Yamada Y. Production of tropane alkaloids in genetically engineered root cultures. Pyhtochemistry 1993;32:713-8.  Back to cited text no. 30
31.Jennewein S, Rithner CD, Williams RM, Croteau RB. Taxol biosynthesis: Taxane 13 alpha-hydroxylase is a cytochrome P450-dependent monooxygenase. Proc Natl Acad Sci U S A 2001;98:13595-600.  Back to cited text no. 31
32.Zhang P, Zhou PP, Yu LJ. An endophytic taxol-producing fungus from Taxus media, Cladosporium cladosporioides MD2. Curr Microbiol 2009;59:227-32.  Back to cited text no. 32
33.Li W, Li M, Yang DL, Xu R, Zhang Y. Production of podophyllotaxin by root culture of Podophyllum hexandrum Royle. Electron J Biol 2009;5:34-9.  Back to cited text no. 33
34.Engels B, Dahm P, Jennewein S. Metabolic engineering of taxadiene biosynthesis in yeast as a first step towards Taxol (Paclitaxel) production. Metab Eng 2008;10:201-6.  Back to cited text no. 34
35.Komaraiah P, Ramakrishna SV, Reddanna P, Kavi Kishor PB. Enhanced production of plumbagin in immobilized cells of Plumbago rosea by elicitation and it situ adsorption. J Biotechnol 2003;10:181-7.  Back to cited text no. 35
36.Vanisree M, Chen YL, Shu-Fung L, Satish MN, Chien YL, HsinSheng T. Studies on the production of some important secondary metabolites from medicinal plants by plant tissue cultures. Bott Bull Acad Sin 2004;45:1-22.  Back to cited text no. 36
37.Jordon MA, Wilson L. Microtuble polymerization dynamics, mitotic, and cell death by paclitaxel at low concentration Am Chem Soc Symp Ser 1995;583:138-53.  Back to cited text no. 37
38.Cragg GM, Schepartz SA, Suffness M, Grever MR. The taxol supply crisis. New NCI policies for handling the large-scale production of novel natural product anticancer and anti-HIV agents. J Nat Prod 1993;56:1657-68.  Back to cited text no. 38
39.Suffness, M. Taxol: Science and Applications. Boca Raton, FL: CRC Press; 1995.  Back to cited text no. 39
40.Fett-Neto AG, Stewart JM, Nicholson SA, Pennington JJ, DiCosmo F. Improved taxol yield by aromatic carboxylic acid and and amino acid feeding to cell cultures of T. cuspidata. Biotechnol Bioeng 1994;44:967-71.  Back to cited text no. 40
41.Ciddi V, Srinivasan V, Shuler VM. Elicitation of Taxus cell cultures for production of taxol. Biotechnol Lett 1995;17:1343-6.  Back to cited text no. 41
42.Strobel GA, Stierle A, van Kuijk JG. Factors influencing the in vitro production of radiolabelled taxol by Pacific yew, Taxus brevifolia. Plant Sci 1992;84:65-74.  Back to cited text no. 42
43.Yukimune Y, Tabata H, Higashi Y, Hara Y. Methyljasmonate-induced overproduction of paclitaxel and baccatin III in Taxus cell suspension cultures. Nat Biotechnol 1996;14:1129-32.  Back to cited text no. 43
44.Tam WH, Constabel F, Kurz WG. Codeine from cell suspension cultures of Papaver somniferum. Phytochemistry 1980;19:486-7.  Back to cited text no. 44
45.Yoshikawa T, Furuya T. Morphinan alkaloid production by tissues differentiated from cultured cells of Papaver somniferum (1). Planta Med 1985;2:110-3.  Back to cited text no. 45
46.Furuya T, Ikuta A, Syono K. Alkaloids from callus cultures of Papaver somniferum. Phytochemistry 1972;11:3041-4.  Back to cited text no. 46
47.Guggenheim M. Dioxyphenylalanin, eine neue Aminosaure aus Vinca faba. Z Physiol Chem 1913;88:276.  Back to cited text no. 47
48.Daxenbichler ME, VanEtten CH, Hallinan EA, Earle FR, Barclay AS. Seeds as sources of L-DOPA. J Med Chem 1971;14:463-5.  Back to cited text no. 48
49.Brain KR, Lockwood GB. Hormonal control of steroid levels in tissue cultures from Trigonella foenumgraecum. Phytochemistry 1976;15:1651-4.  Back to cited text no. 49
50.Tal B, Rokem JS, Goldberg I. Factors affecting growth and product formation in plant cells grown in continuous culture. Plant Cell Rep 1983;2:219-22.  Back to cited text no. 50
51.Zenk MH, el-Shagi H, Schulte U. Anthraquinone production by cell suspension cultures of Morinda citrifolia. Planta Med 1978;Suppl:79-101.  Back to cited text no. 51
52.Ravishankar GA, Suresh B, Giridhar P, Rao SR, Johnson TS. 2003 Biotechnological studies on capsicum for metabolite production and plant improvement. In: DE, Amit Krishna ed. Capsicum: The genus Capsicum. UK: Harwood Academic Publishers; 2003. p. 96-128.  Back to cited text no. 52
53.Sharma A, Kumar V, Giridhar P, Ravishankar GA. Induction of in vitro flowering in Capsicum frutescens under the influence of silver nitrate and cobalt chloride and pollen transformation. Plant Biotechnol J 2008;11: full text-8.  Back to cited text no. 53
54.Lindsey K. Manipulation by nutrient limitation of the biosynthetic activity of immobilized cells of Capsicum frutescens Mill. ev. annum. Planta 1985;165:126-33.  Back to cited text no. 54
55.Holden RR, Holden MA, Yeoman MM. The effects of fungal elicitation on secondary metabolism in cell cultures of Capsicum frutescens. In: RJ Robins, MJC Rhodes, editors. Manipulating secondary metabolism in culture. Cambridge, England: Cambridge University Press; 1988. p. 67-72.  Back to cited text no. 55
56.Ravishankar GA, Ramachandra Rao. Biotechnological production of phytopharmaceuticals. J Biochem Mol Biol Biophys 2000;4:73-102.  Back to cited text no. 56
57.Sanatombi K, Sharma GJ. Micropropagation of Capsicum frutescens L. using axillary shoot explants. Sci Hortic 2007;113:96-9.  Back to cited text no. 57
58.Padmanabha BV, Chandrashekar M, Ramesha BT, Hombe Gowda HC, Rajesh PG, Suhas S, et al. Patterns of accumulation of camptothecin, an anti-cancer alkaloids in Nothapodytes nimmoniana Graham, in the Western Ghats, India: Implications for identifying high-yielding sources of the alkaloid. Curr Sci 2006;90:95-100.  Back to cited text no. 58
59.Sakato K, Misawa M. Effects of chemical and physical conditions on growth of Camptotheca acuminata cell cultures. Agri Biol Chem 1974;38:491-7.  Back to cited text no. 59
60.Thengane SR, Kulkarni DK, Shrikhande VA, Joshi SP, Sonawane KB, Krishnamurthy KV. Influence of medium composition on callus induction and camptothecin(s) accumulation in Nothapodytes foetida. Plant Cell Tiss Org Cult 2003;72:247-51.  Back to cited text no. 60
61.Vanisree M, Tsay HS. Plant Cell Cultures - An Alternative and Efficient Source for the Production of Biologically Important Secondary Metabolites. International J Appl Sci Engin 2004;2:29-48.   Back to cited text no. 61
62.Breuling M, Alfermann AW, Reinhard E. Cultivation of cell cultures of Berberis wilsonae in 20l airlift bioreactors. Plant Cell Rep 1985;4:220-3.  Back to cited text no. 62
63.Sato F, Yamada Y. High berberine producing cultures of Coptis japonica cells. Phytochemistry 1984;23:281-5.  Back to cited text no. 63
64.Nakagawa K, Konagai A, Fukui H, Tabata M. Release and crystalization of berberine in the liquid medium of Thalictrum minus cell suspension cultures. Plant Cell Rep 1984;3:254-7.  Back to cited text no. 64
65.Suzuki M, Nakagawa K, Fukui H, Tabata M. Alkaloid production in cell suspension cultures of Thalictrum flavum and T. dipterocarpum. Plant Cell Rep 1988;7:26-9.  Back to cited text no. 65
66.Nakagawa K, Fukui H, Tabata M. Hormonal regulation of berberine production in cell suspension cultures of Thalictrum minus. Plant Cell Rep 1986;5:69-71.  Back to cited text no. 66
67.Morimoto T, Hara Y, Kato Y, Hiratsuka J, Yoshioka T, Fujita Y, et al. Berberine production by cultured Coptis japonica cells in one-stage culture using medium with a high copper concentration. Agri Biol Chem 1988;52:1835-6.  Back to cited text no. 67
68.Funk C, Gugler K, Brodelius P. Increased secondary product formation in plant cell suspension cultures after treatment with a yeast carbohydrate preparation (elicitor). Phytochemistry 1987;26:401-5.  Back to cited text no. 68
69.Hara M, Kobayashi Y, Fukui H, Tabata M. Enhancement of berberine production by spermidine in Thalictrum minus cell suspension cultures. Plant Cell Rep 1991;10:494-7.  Back to cited text no. 69
70.Lessard P. Metabolic engineering: the concept coalesces. Nat Biotechnol 1996;14:1654-5.  Back to cited text no. 70
71.Kinney AJ. Manipulating flux through plant metabolic pathways. Curr Opin Plant Biol 1998;1:173-8.  Back to cited text no. 71
72.Sato F, Hashimoto T, Hachiya A, Tamura K, Choi KB, Morishige T, et al. Metabolic engineering of plant alkaloid biosynthesis. Proc Natl Acad Sci U S A 2001;2:367-72.  Back to cited text no. 72
73.Yan A, Kohli A, Koffas MA. Biosynthesis of natural flavanones in Saccharomyces cerevisiae. Appl Environ Microbiol 2005;71:5610-3.  Back to cited text no. 73
74.Oswald M, Fischer M, Dirninger N, Karst F. Monoterpenoid biosynthesis in Saccharomyces cerevisiae. FEMS Yeast Res 2007;7:413-21.   Back to cited text no. 74
75.Ro DK, Paradise EM, Ouellet M. Production of the anti-malarial drug precursor artemisinic acid in engineered yeast. Nature 2007;440:940-4.  Back to cited text no. 75
76.Gunel T, Kuntz M, Arda N, Erturk S, Temizkan G. Metabolic engineering for production of geranylgeranyl pyrophosphate synthase in non-carotenogenic yeast Schizosaccharomyces pombe. Biotechnol Biotechnol Eq 2006;20:76-82.  Back to cited text no. 76
77.Geerlings A, Redondo FJ, Contin A, Memelink J, van der Heijden R, Verpoorte R. Biotransformation of tryptamine and secologanin into plant terpenoid indole alkaloids by transgenic yeast. Appl Microbiol Biotechnol 2001;56:420-4.  Back to cited text no. 77
78.Anderson LA, Roberts MF, Phillipson JD. Studies on Ailanthus altissima cell suspension cultures. The effect of basal media on growth and alkaloid production. Plant Cell Rep 1987;6:239-41.   Back to cited text no. 78
79.Anderson LA, Hay CA, Roberts MF, Phillipson JD. Studies on Ailanthus altissima cell suspension cultures. Plant Cell Rep 1986;5:387-90.  Back to cited text no. 79
80.Malpathak NP, David SB. Flavor formation in tissue cultures of garlic (Allium sativum L.) Plant Cell Rep 1986;5:446-7.  Back to cited text no. 80
81.Yagi A, Shoyama Y, Nishioka I. Formation of tetrahydroanthrcence glucosides by callus tissue of Aloe saponaria. Phytochemistry 1983;22:1483-4.  Back to cited text no. 81
82.Goleniowski M, Tirppi VS. Effect of growth medium composition on psilostachyinolides and altamisine production. Plant Cell Tiss Org Cult 1999;56:215-8.  Back to cited text no. 82
83.Wang PJ, Huang CI. Production of saikosaponins by callus and redifferentiated organs of Bupleurum falcatum L. In: Fujiwara, editor. Plant Tissue Culture. Tokyo: Maruzen; 1982. p. 71-2.  Back to cited text no. 83
84.Nazif NM, Rady MR, el-Nasr MM. Stimulation of anthraquinone production in suspension cultures of Cassia acutifolia by salt stress. Fitoterapia 2000;71:34-40.  Back to cited text no. 84
85.Moreno PR, Heijden RV, Verpoorte R. Effect of terpenoid precursor feeding and elicitation on formation of indole alkaloids in cell suspension cultures of Catharanthus roseus. Plant Cell Rep 1993;12:702-5.  Back to cited text no. 85
86.Zhao J, Zhu W, Hu Q. Enhanced catharanthine production in Catharanthus roseus cell cultures by combined elicitor treatment in shake flasks and bioreactors. Enzyme Microb Technol 2001;28:673-81.  Back to cited text no. 86
87.Barthe GA, Jourdan PS, McIntosh CA, Mansell RL. Naringin and limonin production in callus culture and regenerated shoots from Citrus sp. J Plant Physiol 1987;127:55-65.  Back to cited text no. 87
88.Waller GR, Mac Vean CD, Suzuki T. High production of caffeine and related enzyme activities in callus cultures of Coffea arabica L. Plant Cell Rep 1983;2:109-12.  Back to cited text no. 88
89.Iwasa K, Takao N. Formation of alkaloids in Corydalis ophiocarpa callus cultures. Phytochemistry 1982;21:611-4.  Back to cited text no. 89
90.Morimoto H, Murai F. The effect of gelling agents on planuotol accumulation in callus cultures of Croton sublyratus Kurz. Plant Cell Rep 1989;8:210-3.  Back to cited text no. 90
91.Dornenburg H, Knorr D. Semicontinuous processes for anthraquinone production with immobilized Cruciata glabra cell cultures in a three-phase system. J Biotechnol 1999;50:55-62.  Back to cited text no. 91
92.Huang WW, Cheng CC, Yeh FT, Tsay HS. Tissue culture of Dioscorea doryophora HANCE 1. Callus organs and the measurement of diosgenin content. Chin Med Coll J 1993;2:151-60.  Back to cited text no. 92
93.O'Dowd N, McCauley PG, Richardson DH, Wilson G. Callus production, suspension culture and in vitro alkaloid yields of Ephedra. Plant Cell Tiss Org Cult 1993;34:149:55.   Back to cited text no. 93
94.Tanahashi T, Zenk MH. Isoquinoline alkaloids from cell suspension cultures of Fumaria capreolata. Plant Cell Rep 1985;4:96-9.   Back to cited text no. 94
95.Venkateswara R, Sankara Rao, Vaidyanathan CS. Phytochemical constituents of cultured cells of Eucalyptus tereticornis SM. Plant Cell Rep 1986;3:231-3.  Back to cited text no. 95
96.Carrier D, Chauret N, Mancini M, Coulombe P, Neufeld R, Weber M, et al. Detection of ginkgolide A in Ginkgo biloba cell cultures. Plant Cell Rep 1991;10:256-9.  Back to cited text no. 96
97.Ayabe S, Takano H, FujitaT, Hirota H, Takahshi T. Titerpenoid biosynthesis in tissue cultures of Glycyrrhiza glabra var. glandulifera. Plant Cell Rep 1990;9:181-4.  Back to cited text no. 97
98.Arrebola ML, Ringbom T, Verpoorte R. Anthraquinones from Isoplexis isabelliana cell suspension cultures. Phytochemistry 1999;52:1283-6.  Back to cited text no. 98
99.Uden W, Pras N, Vossebeld EM, Mol JN, Malingre TM. Production of 5-methoxypodophyllotoxin in cell suspension cultures of Linum flavum L. Plant Cell Tiss Org Cult 1990;20:81-7.  Back to cited text no. 99
100.Wichers HJ, Visser JF, Huizing HJ, Pras N. Occurrence of L-DOPA and dopamine in plants and cell cultures of Mucuna pruriens and effects of 2,4-D and NaCl on these compounds. Plant Cell Tiss Org Cult 1993;33:259-64.  Back to cited text no. 100
101.Zhong JJ, Zhu QX. Effect of initial phosphate concentration on cell growth and ginsenoside saponin production by suspended cultures of Panax notoginseng. Appl Biochem Biotechnol 1995;55:241-6.  Back to cited text no. 101
102.Baumert A, Groger D, Kuzovkina IN, Reisch J. Secondary metabolites produced by callus cultures of various Ruta species. Plant Cell Tiss Org Cult 1992;28:159-62.  Back to cited text no. 102


  [Figure 1], [Figure 2], [Figure 3], [Figure 4], [Figure 5], [Figure 6]

  [Table 1]

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[Pubmed] | [DOI]
10 Dietary plant pigment on blood-digestive physiology, antioxidant-immune response, and inflammatory gene transcriptional regulation in spotted snakehead (Channa punctata) infected with Pseudomonas aeruginosa
Ramasamy Harikrishnan, Gunapathy Devi, Hien Van Doan, S. Vijay, Chellam Balasundaram, Einar Ringř, Seyed Hossein Hoseinifar, Sanchai Jaturasithaf
Fish & Shellfish Immunology. 2022; 120: 716
[Pubmed] | [DOI]
11 Effects of probiotic consortia on plant metabolites are associated with soil indigenous microbiota and fertilization regimes
Zhikang Wang, Ziyun Chen, Marcio F.A. Leite, Ziheng Xu, Quan Lin, George A. Kowalchuk, Xiangxiang Fu, Eiko E. Kuramae
Industrial Crops and Products. 2022; 185: 115138
[Pubmed] | [DOI]
12 A Comprehensive and Current Review on the Role of Flavonoids in Lung Cancer: Experimental and Theoretical Approaches
Seyda Berk, Savas Kaya, Esra Küpeli Akkol, Hilal Bardakçi
Phytomedicine. 2022; : 153938
[Pubmed] | [DOI]
13 In vitro elicitation of Camptothecin by challenging with biotic elicitors in Nothapodytes nimmoniana (J.Graham) Mabb
B Keshavan, N Santosh Srinivas, M Muthu Tamizh, M Vairamani, Raman Pachaiappan
South African Journal of Botany. 2022; 144: 325
[Pubmed] | [DOI]
14 Systematic enhancement of L-DOPA and Secondary metabolites from Mucuna imbricata: Implication of precursors and elicitors in Callus culture
Suresh Suryawanshi, Parthraj Kshirsagar, Prajakta Kamble, Vishwas Bapat, Jyoti Jadhav
South African Journal of Botany. 2022; 144: 419
[Pubmed] | [DOI]
15 A simple validated HPTLC method for the analysis of flavonoids and molecular docking studies of novel tri-terpenoid glycoside isolated from Carya illinoinensis bark with potential anti-inflammatory and antinociceptive activities
Md.Sarfaraj Hussain, Faizul Azam, Jamal Mezogi, Fatima Abdmalla Enwij, Ghazalla M Benhusein, Anzarul Haque, Mohammad Khalid, Muhammed Arif, Mohammad Mahtab Alam, Irfan Ahmad, Mohd. Saeed
South African Journal of Botany. 2022; 147: 596
[Pubmed] | [DOI]
16 Influence of elicitors on the enhancement of camptothecin accumulation and antioxidant potential in callus cultures of Chonemorpha fragrans
N.P. Ambujakshi, T. Ravikiran, H.R. Raveesha
South African Journal of Botany. 2022; 150: 225
[Pubmed] | [DOI]
17 Exploiting plant transcriptomic databases: Resources, tools, and approaches
Peng Ken Lim, Xinghai Zheng, Jong Ching Goh, Marek Mutwil
Plant Communications. 2022; : 100323
[Pubmed] | [DOI]
18 Microwave Radiation as an Inducer of Secondary Metabolite Production in Drosera rotundifolia In Vitro Plantlets
Antonio López-Orenes, María A. Ferrer, Antonio A. Calderón
Journal of Natural Products. 2022;
[Pubmed] | [DOI]
19 Effect of Light Quality and Media Components on Shoot Growth, Rutin, and Quercetin Production from Common Buckwheat
Ahmed M. M. Gabr, Nesrin M. Fayek, Hossam M. Mahmoud, Mohamed K. El-Bahr, Hanan S. Ebrahim, Oksana Sytar, Ali M. El-Halawany
ACS Omega. 2022;
[Pubmed] | [DOI]
20 Growth dynamics and differential accumulation of picrosides and its precursor metabolites in callus cell lines of Picrorhiza kurroa with distinct anti-steatotic potential
Mahinder Partap, Jyoti Chhimwal, Pawan Kumar, Dinesh Kumar, Yogendra Padwad, Ashish R. Warghat
Process Biochemistry. 2022; 120: 85
[Pubmed] | [DOI]
21 Cambial meristematic cell culture: a sustainable technology toward in vitro specialized metabolites production
Mahinder Partap, Ashish R. Warghat, Sanjay Kumar
Critical Reviews in Biotechnology. 2022; : 1
[Pubmed] | [DOI]
22 Fungal biotransformation of carvone and camphor by Aspergillus flavus and investigation of cytotoxic activities of naturally obtained essential oils
Isil Gazioglu, Ozge Sultan Zengin, Aysenur Gunaydin Akyildiz, Belma Zengin Kurt
Natural Product Research. 2022; : 1
[Pubmed] | [DOI]
23 Genome-wide identification and characterization of active ingredients related ß-Glucosidases in Dendrobium catenatum
Zhicai Wang, Meili Zhao, Xiaojie Zhang, Xuming Deng, Jian Li, Meina Wang
BMC Genomics. 2022; 23(1)
[Pubmed] | [DOI]
24 In vitro strategies for the enhancement of secondary metabolite production in plants: a review
Mohammad Afaan Fazili, Irfan Bashir, Mudasar Ahmad, Ubaid Yaqoob, Syed Naseem Geelani
Bulletin of the National Research Centre. 2022; 46(1)
[Pubmed] | [DOI]
25 Tailoring enhanced production and identification of isoflavones in the callus cultures of Pueraria thomsonii Benth and its model verification using response surface methodology (RSM): a combined in vitro and statistical optimization
Yu Li, Pachaiyappan Saravana Kumar, Yu Liu, Jiao Qiu, Yalan Ran, Mingyuan Yuan, Xinyue Fang, Xuhui Tan, Renjun Zhao, Ji zhu, Meijun He
Beni-Suef University Journal of Basic and Applied Sciences. 2022; 11(1)
[Pubmed] | [DOI]
26 The Transcriptome Profiling of Flavonoids and Bibenzyls Reveals Medicinal Importance of Rare Orchid Arundina graminifolia
Sagheer Ahmad, Jie Gao, Yonglu Wei, Chuqiao Lu, Genfa Zhu, Fengxi Yang
Frontiers in Plant Science. 2022; 13
[Pubmed] | [DOI]
27 Evaluation of the effect of jasmonic acid elicitation on composition of pigments and biological activities in green callus of neem (Azadirachta indica)
Nurul Syazwani Ahmad Fauzi, Muhamad Hafiz Abd Rahim, Nazia Abdul Majid, Rashidi Othman, Jamilah Syafawati Yaacob
Frontiers in Sustainable Food Systems. 2022; 6
[Pubmed] | [DOI]
28 Establishment of Stem Cell-like Cells of Sida hermaphrodita (L.) Rusby from Explants Containing Cambial Meristems
Šarlota Kanuková, Marcela Gubišová, Lenka Klcová, Daniel Mihálik, Ján Kraic
International Journal of Molecular Sciences. 2022; 23(14): 7644
[Pubmed] | [DOI]
29 Metabolic Engineering of Escherichia coli for Hyperoside Biosynthesis
Guosi Li, Fucheng Zhu, Peipei Wei, Hailong Xue, Naidong Chen, Baowei Lu, Hui Deng, Cunwu Chen, Xinjian Yin
Microorganisms. 2022; 10(3): 628
[Pubmed] | [DOI]
30 Genetic Manipulation and Bioreactor Culture of Plants as a Tool for Industry and Its Applications
Tomasz Kowalczyk, Anna Merecz-Sadowska, Laurent Picot, Irena Brcic Karaconji, Joanna Wieczfinska, Tomasz Sliwinski, Przemyslaw Sitarek
Molecules. 2022; 27(3): 795
[Pubmed] | [DOI]
31 Effect of Ethylene and Abscisic Acid on Steroid and Triterpenoid Synthesis in Calendula officinalis Hairy Roots and Saponin Release to the Culture Medium
Michal Markowski, Abdulwadood Shakir Mahmood Alsoufi, Anna Szakiel, Marek Dlugosz
Plants. 2022; 11(3): 303
[Pubmed] | [DOI]
32 Use of Different Organic Carbon Sources in Cynara cardunculus Cells: Effects on Biomass Productivity and Secondary Metabolites
Maria Oliviero, Antonio Luca Langellotti, Giovanni L. Russo, Marco Baselice, Andrea Donadio, Alberto Ritieni, Giulia Graziani, Paolo Masi
Plants. 2022; 11(5): 701
[Pubmed] | [DOI]
33 Phytochemical Screening, Nutritional Value, Anti-Diabetic, Anti-Cancer, and Anti-Bacterial Assessment of Aqueous Extract from Abelmoschus esculentus Pods
Salman Khan, Zeeshan Rafi, Abu Baker, Ambreen Shoaib, Ali G. Alkhathami, Mohammed Asiri, Mohammad Y. Alshahrani, Irfan Ahmad, Yasser Alraey, Ali Hakamy, Mohd Saeed, Shazia Mansoor
Processes. 2022; 10(2): 183
[Pubmed] | [DOI]
34 Polish Achievements in Bioactive Compound Production From In Vitro Plant Cultures
Agnieszka Pietrosiuk, Anna Budzianowska, Jaromir Budzianowski, Halina Ekiert, Malgorzata Jeziorek, Anna Kawiak, Malgorzata Kikowska, Miroslawa Krauze-Baranowska, Aleksandra Królicka, Lukasz Kuzma, Maria Luczkiewicz, Janusz Malarz, Adam Matkowski, Anna Stojakowska, Katarzyna Syklowska-Baranek, Agnieszka Szopa, Wojciech Szypula, Barbara Thiem, Sylwia Zielinska, Robert Konieczny
Acta Societatis Botanicorum Poloniae. 2022; 91
[Pubmed] | [DOI]
35 The Bark of the Spruce Picea jezoensis Is a Rich Source of Stilbenes
Andrey R. Suprun, Alexandra S. Dubrovina, Olga A. Aleynova, Konstantin V. Kiselev
Metabolites. 2021; 11(11): 714
[Pubmed] | [DOI]
36 Antibacterial potency of extracted essential oils of some plant species against common gram-positive and gram-negative bacteria
GaffarSarwar Zaman
King Khalid University Journal of Health Sciences. 2021; 6(1): 18
[Pubmed] | [DOI]
37 Role of Traditional Ethnobotanical Knowledge and Indigenous Communities in Achieving Sustainable Development Goals
Ajay Kumar, Sushil Kumar, Komal, Nirala Ramchiary, Pardeep Singh
Sustainability. 2021; 13(6): 3062
[Pubmed] | [DOI]
38 Biosynthesis of trifolin, a bioactive flavonoid by biotransformation
Hye-Ryeong Noh, Ju-Yeong Kang, Bong-Gyu Kim
Journal of Applied Biological Chemistry. 2021; 64(3): 309
[Pubmed] | [DOI]
39 Transcriptomic Analyses Shed Light on Critical Genes Associated with Bibenzyl Biosynthesis in Dendrobium officinale
Oluwaniyi Isaiah Adejobi, Ju Guan, Liu Yang, Jiang-Miao Hu, Anmin Yu, Sammy Muraguri, Aizhong Liu
Plants. 2021; 10(4): 633
[Pubmed] | [DOI]
40 Development of a Cell Suspension Culture System for Promoting Alkaloid and Vinca Alkaloid Biosynthesis Using Endophytic Fungi Isolated from Local Catharanthus roseus
Tran My Linh, Nguyen Chi Mai, Pham Thi Hoe, Ninh Thi Ngoc, Phan Thi Hong Thao, Ninh Khac Ban, Nguyen Tuong Van
Plants. 2021; 10(4): 672
[Pubmed] | [DOI]
41 Comparative Effects of Different Light Sources on the Production of Key Secondary Metabolites in Plants In Vitro Cultures
Mariam Hashim, Bushra Ahmad, Samantha Drouet, Christophe Hano, Bilal Haider Abbasi, Sumaira Anjum
Plants. 2021; 10(8): 1521
[Pubmed] | [DOI]
42 A Comprehensive Study of the Antibacterial Activity of Bioactive Juice and Extracts from Pomegranate (Punica granatum L.) Peels and Seeds
Kaja Kupnik, Mateja Primožic, Katja Vasic, Željko Knez, Maja Leitgeb
Plants. 2021; 10(8): 1554
[Pubmed] | [DOI]
43 Enhancement of Phytosterol and Triterpenoid Production in Plant Hairy Root Cultures—Simultaneous Stimulation or Competition?
Agata Rogowska, Anna Szakiel
Plants. 2021; 10(10): 2028
[Pubmed] | [DOI]
44 Boosting of Antioxidants and Alkaloids in Catharanthus roseus Suspension Cultures Using Silver Nanoparticles with Expression of CrMPK3 and STR Genes
Ahmed Fouad, Adel E. Hegazy, Ehab Azab, Ebtihal Khojah, Tarek Kapiel
Plants. 2021; 10(10): 2202
[Pubmed] | [DOI]
45 MTMS-Based Aerogel Constructs for Immobilization of Plant Hairy Roots: Effects on Proliferation of Rindera graeca Biomass and Extracellular Secretion of Naphthoquinones
Bartosz Nowak, Mateusz Kawka, Kamil Wierzchowski, Katarzyna Syklowska-Baranek, Maciej Pilarek
Journal of Functional Biomaterials. 2021; 12(1): 19
[Pubmed] | [DOI]
46 Effect of the Carbon Source and Plant Growth Regulators (PGRs) in the Induction and Maintenance of an In Vitro Callus Culture of Taraxacum officinale (L) Weber Ex F.H. Wigg
María Eugenia Martinez, Lorena Jorquera, Paola Poirrier, Katy Díaz, Rolando Chamy
Agronomy. 2021; 11(6): 1181
[Pubmed] | [DOI]
47 Plant in vitro Culture Technologies; A Promise Into Factories of Secondary Metabolites Against COVID-19
Tariq Khan, Mubarak Ali Khan, Kashmala Karam, Nazif Ullah, Zia-ur-Rehman Mashwani, Akhtar Nadhman
Frontiers in Plant Science. 2021; 12
[Pubmed] | [DOI]
48 Mass propagation of Juniperus procera Hoechst. Ex Endl. From seedling and screening of bioactive compounds in shoot and callus extract
Abdalrhaman M. Salih, Fahad Al-Qurainy, Salim Khan, Mohamed Tarroum, Mohammad Nadeem, Hassan O. Shaikhaldein, Nadiyah M. Alabdallah, Saleh Alansi, Aref Alshameri
BMC Plant Biology. 2021; 21(1)
[Pubmed] | [DOI]
49 Perspectives of future water sources in Qatar by phytoremediation: biodiversity at ponds and modern approach
R. F. Al-Thani, B. T. Yasseen
International Journal of Phytoremediation. 2021; 23(8): 866
[Pubmed] | [DOI]
50 Biomass production and secondary metabolite identification in callus cultures of Coryphantha macromeris (Engelm.) Britton & Rose (Cactaceae), a traditional medicinal plant
Emmanuel Cabańas-García, Carlos Areche, Yenny Adriana Gómez-Aguirre, Jorge Borquez, Ruben Muńoz, Francisco Cruz-Sosa, Eugenio Pérez-Molphe Balch
South African Journal of Botany. 2021; 137: 1
[Pubmed] | [DOI]
51 Variation of eurycomanone content within and among populations of E. apiculata A.W. Benn.
Zulfahmi, Parjanto, E Purwanto, B Pujiasmanto, A T Sakya, Samanhudi, Rosmaina, A Yunus
IOP Conference Series: Earth and Environmental Science. 2021; 905(1): 012080
[Pubmed] | [DOI]
52 Using Stress Factors for Storage of Withania somnifera L. Hairy Roots without Passages
K. G. Musin, G. R. Gumerova, E. Gorte, E. A. Baimukhametova, E. V. Mikhaylova, B. R. Kuluev
Russian Journal of Plant Physiology. 2021; 68(3): 536
[Pubmed] | [DOI]
53 Comparative Cytotoxic Activity of Wild Harvested Stems and In Vitro-Raised Protocorms of Dendrobium chryseum Rolfe in Human Cervical Carcinoma and Glioblastoma Cell Lines
Bijaya Pant, Pusp Raj Joshi, Sabitri Maharjan, Laxmi Sen Thakuri, Shreeti Pradhan, Sujit Shah, Sven H. Wagner, Basant Pant, Ergin Murat Altuner
Advances in Pharmacological and Pharmaceutical Sciences. 2021; 2021: 1
[Pubmed] | [DOI]
54 UV-C mediated accumulation of pharmacologically significant phytochemicals under light regimes in in vitro culture of Fagonia indica (L.)
Bilal Haider Abbasi, Taimoor Khan, Razia Khurshid, Muhammad Nadeem, Samantha Drouet, Christophe Hano
Scientific Reports. 2021; 11(1)
[Pubmed] | [DOI]
55 The impact of carbon source on cell growth and the production of bioactive compounds in cell suspensions of Hancornia speciosa Gomes
Luciana Arantes Dantas, Paula Sperotto Alberto Faria, Bruno Matheus Mendes Dário, Ana Luíza Martins Arantes, Fabiano Guimarăes Silva, Roniel Geraldo Avila, Paulo Sérgio Pereira, Aurélio Rubio Neto
Scientific Reports. 2021; 11(1)
[Pubmed] | [DOI]
56 Biosynthesis of zinc oxide nanoparticles using Phoenix dactylifera and their effect on biomass and phytochemical compounds in Juniperus procera
Abdalrhaman M. Salih, Fahad Al-Qurainy, Salim Khan, Mohamed Tarroum, Mohammad Nadeem, Hassan O. Shaikhaldein, Abdel-Rhman Zakaria Gaafar, Norah S. Alfarraj
Scientific Reports. 2021; 11(1)
[Pubmed] | [DOI]
57 Functional aspects of plant secondary metabolites in metal stress tolerance and their importance in pharmacology
K.S. Anjitha, P.P. Sameena, Jos T. Puthur
Plant Stress. 2021; 2: 100038
[Pubmed] | [DOI]
58 Natural rubber-producing sources, systems, and perspectives for breeding and biotechnology studies of Taraxacum kok-saghyz
Maryam Salehi, Katrina Cornish, Moslem Bahmankar, Mohammad Reza Naghavi
Industrial Crops and Products. 2021; 170: 113667
[Pubmed] | [DOI]
59 Cryopreservation of plant cell cultures – Diverse practices and protocols
Henrik Nausch, Johannes F. Buyel
New Biotechnology. 2021; 62: 86
[Pubmed] | [DOI]
60 Secondary metabolites in the drought stress tolerance of crop plants: A review
Bindu Yadav, Abhimanyu Jogawat, Md Samiur Rahman, Om Prakash Narayan
Gene Reports. 2021; 23: 101040
[Pubmed] | [DOI]
61 Evaluation of bioactive flavonols and ent-kaurenes in the 2,4-dichlorophenoxyacetic acid-induced calli of Senegalia nigrescens using FTIR and GC–MS
Olusola Bodede, Shakira Shaik, Roshila Moodley
Journal of Plant Biochemistry and Biotechnology. 2021;
[Pubmed] | [DOI]
62 Multi-fold enhancement in vitamin E (alpha-tocopherol) production via integration of bioprocess optimisation and metabolic engineering in cell suspension of sunflower
Aparajitha Srinivasan, Vijayakumar Sundaram, M. Vidya Muthulakshmi, Smita Srivastava
Journal of Plant Biochemistry and Biotechnology. 2021;
[Pubmed] | [DOI]
63 Micropropagation, antioxidant and anticancer activity of pineapple orchid: Dendrobium densiflorum Lindl
Bijaya Pant, Krishna Chand, Mukti Ram Paudel, Pusp Raj Joshi, Bir Bahadur Thapa, So Young Park, Sony Shakya, Laxmi Sen Thakuri, Sabari Rajbahak, Anil Kumar Sah, Manju Kanu Baniya, Prithivi Raj Gurung, Lasta Maharjan, Pravesh Rajbhandari
Journal of Plant Biochemistry and Biotechnology. 2021;
[Pubmed] | [DOI]
64 LC–MS untargeted metabolomics assesses the delayed response of glufosinate treatment of transgenic glufosinate resistant (GR) buffalo grasses (Stenotaphrum secundatum L.)
Siriwat Boonchaisri, Simone Rochfort, Trevor Stevenson, Daniel A. Dias
Metabolomics. 2021; 17(3)
[Pubmed] | [DOI]
65 Unveiling microbial community structure in Ragi tape as elicitors to increase secondary metabolites contents in Glycine max and Vigna radiata
Feri E. Hermanto, Warsito Warsito, Muhaimin Rifa’i, Nashi Widodo, Yoga D. Jatmiko
Biologia. 2021;
[Pubmed] | [DOI]
66 Natural Quinone Dyes: A Review on Structure, Extraction Techniques, Analysis and Application Potential
Benson Dulo, Kim Phan, John Githaiga, Katleen Raes, Steven De Meester
Waste and Biomass Valorization. 2021; 12(12): 6339
[Pubmed] | [DOI]
67 Sequencing, de novo assembly and annotation of Digitalis ferruginea subsp. schischkinii transcriptome
Ercan Selçuk Ünlü, Özge Kaya, Ismail Eker, Ekrem Gürel
Molecular Biology Reports. 2021; 48(1): 127
[Pubmed] | [DOI]
68 Organogenesis from Leaf Tissue of Spondias pinnata (L. f.) Kurz, SEM study and Genetic Fidelity Assessment by ISSR and ScoT
Pooja Jaiswal, Nishi Kumari, Sarvesh Pratap Kashyap, Shailesh Kumar Tiwari
Plant Cell, Tissue and Organ Culture (PCTOC). 2021; 146(1): 203
[Pubmed] | [DOI]
69 Elicitation of antioxidant metabolites in Musa species in vitro shoot culture using sucrose, temperature and jasmonic acid
Ibukun O. Ayoola-Oresanya, Mubo A. Sonibare, Badara Gueye, Michael T. Abberton, Gertrud E. Morlock
Plant Cell, Tissue and Organ Culture (PCTOC). 2021; 146(2): 225
[Pubmed] | [DOI]
70 L-phenylalanine applications and culture duration affect root growth and production of tropane alkaloids and phenolics in adventitious root cultures of Hyoscyamus niger L.
Tunhan Demirci, Ilknur Albayrak, Nilgün Göktürk Baydar
Plant Cell, Tissue and Organ Culture (PCTOC). 2021;
[Pubmed] | [DOI]
71 Enhancement of 1-deoxynojirimycin production in mulberry (Morus spp.) using LED irradiation
Marisa Sonthisut, Ratree Wongpanya, Anan Phonphoem, Wannarat Pornsiriwong Phonphoem
Plant Cell, Tissue and Organ Culture (PCTOC). 2021;
[Pubmed] | [DOI]
72 Chemical and real-time based analysis revealed active gene machinery of glycyrrhizin biosynthesis and its accumulation in the aerial tissues of in-vitro regenerated Glycyrrhiza glabra L.
Malik Muzafar Manzoor, Pooja Goyal, Ajai P. Gupta, Saima Khan, Priya Jaswal, Prashant Misra, Pankaj Pandotra, Ashok Ahuja, Ram A. Vishwakarma, Suphla Gupta
Plant Growth Regulation. 2020; 92(2): 263
[Pubmed] | [DOI]
73 Biosynthesis and metabolic actions of simple phenolic acids in plants
Rogério Marchiosi, Wanderley Dantas dos Santos, Rodrigo Polimeni Constantin, Rogério Barbosa de Lima, Anderson Ricardo Soares, Aline Finger-Teixeira, Thatiane Rodrigues Mota, Dyoni Matias de Oliveira, Marcela de Paiva Foletto-Felipe, Josielle Abrahăo, Osvaldo Ferrarese-Filho
Phytochemistry Reviews. 2020; 19(4): 865
[Pubmed] | [DOI]
74 Steviol glycosides profile in Stevia rebaudiana Bertoni hairy roots cultured under oxidative stress-inducing conditions
Marta Libik-Konieczny, Zaneta Michalec-Warzecha, Michal Dziurka, Olga Zastawny, Robert Konieczny, Piotr Rozpadek, Laura Pistelli
Applied Microbiology and Biotechnology. 2020; 104(13): 5929
[Pubmed] | [DOI]
75 Biotic elicitors enhance diosgenin production in Helicteres isora L. suspension cultures via up-regulation of CAS and HMGR genes
Samrin Shaikh, Varsha Shriram, Tushar Khare, Vinay Kumar
Physiology and Molecular Biology of Plants. 2020; 26(3): 593
[Pubmed] | [DOI]
76 Growth Kinetics, Metabolites Production and Expression Profiling of Picrosides Biosynthetic Pathway Genes in Friable Callus Culture of Picrorhiza kurroa Royle ex Benth
Mahinder Partap, Pankaj Kumar, Ashrita, Pawan Kumar, Dinesh Kumar, Ashish R. Warghat
Applied Biochemistry and Biotechnology. 2020; 192(4): 1298
[Pubmed] | [DOI]
77 Schisandra henryi C. B. Clarke in vitro cultures: a promising tool for the production of lignans and phenolic compounds
Karolina Jafernik, Agnieszka Szopa, Magda Barnas, Michal Dziurka, Halina Ekiert
Plant Cell, Tissue and Organ Culture (PCTOC). 2020; 143(1): 45
[Pubmed] | [DOI]
78 Effective callus induction and plant regeneration in callus and protoplast cultures of Nigella damascena L.
Magdalena Klimek-Chodacka, Dariusz Kadluczka, Aneta Lukasiewicz, Aneta Malec-Pala, Rafal Baranski, Ewa Grzebelus
Plant Cell, Tissue and Organ Culture (PCTOC). 2020; 143(3): 693
[Pubmed] | [DOI]
79 Micropropagation and essential oil characterization of Plectranthus amboinicus (Lour.) Sprengel, an aromatic medicinal plant
Greetha Arumugam, Uma Rani Sinniah, Mallappa Kumara Swamy, Paul T. Lynch
In Vitro Cellular & Developmental Biology - Plant. 2020; 56(4): 491
[Pubmed] | [DOI]
80 Elicitation and enhancement of bacoside production using suspension cultures of Bacopa monnieri (L.) Wettst
Anuja Koul, Sharada Mallubhotla
3 Biotech. 2020; 10(6)
[Pubmed] | [DOI]
81 Cadmium chloride (CdCl2) elicitation improves reserpine and ajmalicine yield in Rauvolfia serpentina as revealed by high-performance thin-layer chromatography (HPTLC)
Nadia Zafar, A. Mujib, Muzamil Ali, Dipti Tonk, Basit Gulzar, Moien Qadir Malik, Jyoti Mamgain, Rukaya Sayeed
3 Biotech. 2020; 10(8)
[Pubmed] | [DOI]
82 Foliar application of elicitors enhanced the yield of withanolide contents in Withania somnifera (L.) Dunal (variety, Poshita)
Manali Singh, Nitesh Kumar Poddar, Dipti Singh, Sanjeev Agrawal
3 Biotech. 2020; 10(4)
[Pubmed] | [DOI]
83 Anti-inflammatory, analgesic and molecular docking studies of Lanostanoic acid 3-O-a-D-glycopyranoside isolated from Helichrysum stoechas
Md. Sarfaraj Hussain, Faizul Azam, Hanan Ahmed Eldarrat, Ismail Alkskas, Jamal Abdurahman Mayoof, Jamal Mohammed Dammona, Hend Ismail, Mohammed Ali, Muhammad Arif, Anzarul Haque
Arabian Journal of Chemistry. 2020; 13(12): 9196
[Pubmed] | [DOI]
84 Plant tissue culture as a perpetual source for production of industrially important bioactive compounds
Hema Chandran, Mukesh Meena, Tansukh Barupal, Kanika Sharma
Biotechnology Reports. 2020; 26: e00450
[Pubmed] | [DOI]
85 Elevation of secondary metabolites production through light-emitting diodes (LEDs) illumination in protocorm-like bodies (PLBs) of Dendrobium hybrid orchid rich in phytochemicals with therapeutic effects
Lit Chow Yeow, Bee Lynn Chew, Subramaniam Sreeramanan
Biotechnology Reports. 2020; 27: e00497
[Pubmed] | [DOI]
86 Aroma volatiles, phenolic profile and hypoglycaemic activity of Ajuga iva L.
Ameni Khatteli, Mohamed Ali Benabderrahim, Tebra Triki, Ferdaous Guasmi
Food Bioscience. 2020; 36: 100578
[Pubmed] | [DOI]
87 New benzoic acid derivatives from Cassia italica growing in Saudi Arabia and their antioxidant activity
Rwaida A. Al-Haidari, Mai M. Al-Oqail
Saudi Pharmaceutical Journal. 2020; 28(9): 1112
[Pubmed] | [DOI]
88 Use of plant growth promoting bacteria as an efficient biotechnological tool to enhance the biomass and secondary metabolites production of the industrial crop Pelargonium graveolens L'Hér. under semi-controlled conditions
Leila Riahi, Hanene Cherif, Sahar Miladi, Mohamed Neifar, Bilel Bejaoui, Habib Chouchane, Ahmed Slaheddine Masmoudi, Ameur Cherif
Industrial Crops and Products. 2020; 154: 112721
[Pubmed] | [DOI]
89 miRDetect: A combinatorial approach for automated detection of novel miRNA precursors from plant EST data using homology and Random Forest classification
Garima Ayachit, Himanshu Pandya, Jayashankar Das
Genomics. 2020; 112(5): 3201
[Pubmed] | [DOI]
90 Metabolic engineering of Synechocystis sp. PCC 6803 for the production of aromatic amino acids and derived phenylpropanoids
Laura Furelos Brey, Artur J. Wlodarczyk, Jens F. Bang Thřfner, Meike Burow, Christoph Crocoll, Isabella Nielsen, Agnieszka J. Zygadlo Nielsen, Poul Erik Jensen
Metabolic Engineering. 2020; 57: 129
[Pubmed] | [DOI]
91 Efficient Biosynthesis of R-(-)-Linalool through Adjusting the Expression Strategy and Increasing GPP Supply in Escherichia coli
Xun Wang, Jing Wu, Jiaming Chen, Longjie Xiao, Yu Zhang, Fei Wang, Xun Li
Journal of Agricultural and Food Chemistry. 2020; 68(31): 8381
[Pubmed] | [DOI]
92 Determination of the partition coefficient of isoquinoline alkaloids from Chelidonium majus by reversed phase thin layer chromatography
Aleksandra Czeszak, Matylda Resztak, Andrzej Czyrski, Izabela Nowak
New Journal of Chemistry. 2020; 44(18): 7484
[Pubmed] | [DOI]
93 ‘Hairy’ root extracts as source for ‘green’ synthesis of silver nanoparticles and medical applications
Natalia Kobylinska, Anatolij Shakhovsky, Olena Khainakova, Dmytro Klymchuk, Liliya Avdeeva, Yakiv Ratushnyak, Volodymyr Duplij, Nadiia Matvieieva
RSC Advances. 2020; 10(65): 39434
[Pubmed] | [DOI]
94 Agrobacterium rhizogenes-transformed roots of Astragalus membranaceus (Fisch. Ex Link) Bunge as a source of valuable secondary metabolites
Tatyana Novikova, Elena Ambros, Tatyana Zheleznichenko, Yulianna Zaytseva, Yungeree Oyunbileg, E.V. Banaev, M.A. Tomoshevich, Y.G. Zaytseva
BIO Web of Conferences. 2020; 24: 00064
[Pubmed] | [DOI]
95 Precursor- induced bioaccumulation of secondary metabolites and antioxidant activity in suspension cultures of Dendrobium fimbriatum, an orchid of therapeutic importance
Prasenjit Paul, Suman Kumaria
South African Journal of Botany. 2020; 135: 137
[Pubmed] | [DOI]
96 Bacteria as genetically programmable producers of bioactive natural products
Joachim J. Hug, Daniel Krug, Rolf Müller
Nature Reviews Chemistry. 2020; 4(4): 172
[Pubmed] | [DOI]
97 Efficient Production of the Potent Antimicrobial Metabolite “Terrein” From the Fungus Aspergillus terreus
Jyoti Goutam, Ravindra Nath Kharwar, Vinod Kumar Tiwari, Ranjana Singh, Divakar Sharma
Natural Product Communications. 2020; 15(3): 1934578X20
[Pubmed] | [DOI]
98 Isolation and characterization of nutrient dependent pyocyanin from Pseudomonas aeruginosa and its dye and agrochemical properties
Savitha DeBritto, Tanzeembanu D. Gajbar, Praveen Satapute, Lalitha Sundaram, Ramachandra Yarappa Lakshmikantha, Sudisha Jogaiah, Shin-ichi Ito
Scientific Reports. 2020; 10(1)
[Pubmed] | [DOI]
99 Effect of Foliar Applications of Salicylic Acid and Chitosan on the Essential Oil of Thymbra spicata L. under Different Soil Moisture Conditions
Maryam Momeni, Abdollah Ghasemi Pirbalouti, Amir Mousavi, Hassanali Naghdi Badi
Journal of Essential Oil Bearing Plants. 2020; 23(5): 1142
[Pubmed] | [DOI]
100 Subject: UV-B radiation and low temperature promoted hypericin biosynthesis in adventitious root culture of Hypericum perforatum
Farahnaz Tavakoli, Mohammad Rafieiolhossaini, Rudabeh Ravash, Morteza Ebrahimi
Plant Signaling & Behavior. 2020; 15(7): 1764184
[Pubmed] | [DOI]
101 Chemical Composition and Anti-proliferative Activity of Essential Oil from Rhizomes of Micropropagated Curcuma aromatica in Eastern India
Reena Parida, Sujata Mohanty, Sanghamitra Nayak
Journal of Biologically Active Products from Nature. 2020; 10(1): 1
[Pubmed] | [DOI]
102 Time-series transcriptomic analysis reveals novel gene modules that control theanine biosynthesis in tea plant (Camellia sinensis)
Haisheng Cao, Xiaolong He, Jinke Du, Rui Zhang, Ying Chen, Yong Ma, Qi Chen, Congbing Fang, Chi-Tang Ho, Shihua Zhang, Xiaochun Wan, Tapan Kumar Mondal
PLOS ONE. 2020; 15(9): e0238175
[Pubmed] | [DOI]
103 Characterization of (2E,6E)-3,7,11-Trimethyldodeca-2,6,10-Trien-1-Ol with Antioxidant and Antimicrobial Potentials from Euclea Crispa (Thunb.) Leaves
Chella Perumal Palanisamy, Bo Cui, Hong Xia Zhang, Thanh Trung Nguyen, Hoang Dung Tran, Tran Dang Khanh, Van Quan Nguyen, Tran Dang Xuan
International Letters of Natural Sciences. 2020; 80: 51
[Pubmed] | [DOI]
104 Micropropagation of Turkestan Soap Root Allochrusa gypsophiloides – Natural Source of Saponins
International Journal of Secondary Metabolite. 2020; : 1
[Pubmed] | [DOI]
105 Importance of Environmental Factors on Production of Computationally- Defined Natural Molecules against COVID-19 Pandemic
Mohamed Abouleish, Ali El-Keblawy, Kareem A. Mosa, Sameh S.M. Soliman
Current Topics in Medicinal Chemistry. 2020; 20(22): 1958
[Pubmed] | [DOI]
106 Induced extracellular production of stilbenes in grapevine cell culture medium by elicitation with methyl jasmonate and stevioside
Yu Jeong Jeong, Su Hyun Park, Sung-Chul Park, Soyoung Kim, Tae Hee Kim, Jiyoung Lee, Suk Weon Kim, Young Bae Ryu, Jae Cheol Jeong, Cha Young Kim
Bioresources and Bioprocessing. 2020; 7(1)
[Pubmed] | [DOI]
107 A new approach to prevent hazelnut callus browning by modification of sub-culture
Biologia plantarum. 2020; 64: 417
[Pubmed] | [DOI]
108 Elucidating Mechanisms of Endophytes Used in Plant Protection and Other Bioactivities With Multifunctional Prospects
Ayomide Emmanuel Fadiji, Olubukola Oluranti Babalola
Frontiers in Bioengineering and Biotechnology. 2020; 8
[Pubmed] | [DOI]
109 Endophytic Fungi of Marine Alga From Konkan Coast, India—A Rich Source of Bioactive Material
Siya Kamat, Madhuree Kumari, Sidhartha Taritla, C. Jayabaskaran
Frontiers in Marine Science. 2020; 7
[Pubmed] | [DOI]
110 Hairy Root Cultures—A Versatile Tool With Multiple Applications
Noemi Gutierrez-Valdes, Suvi T. Häkkinen, Camille Lemasson, Marina Guillet, Kirsi-Marja Oksman-Caldentey, Anneli Ritala, Florian Cardon
Frontiers in Plant Science. 2020; 11
[Pubmed] | [DOI]
111 Natural Plant Products: A Less Focused Aspect for the COVID-19 Viral Outbreak
Anamika Pandey, Mohd Kamran Khan, Mehmet Hamurcu, Sait Gezgin
Frontiers in Plant Science. 2020; 11
[Pubmed] | [DOI]
112 Plant Secondary Metabolites in the Battle of Drugs and Drug-Resistant Bacteria: New Heroes or Worse Clones of Antibiotics?
Cyrill L. Gorlenko, Herman Yu. Kiselev, Elena V. Budanova, Andrey A. Zamyatnin, Larisa N. Ikryannikova
Antibiotics. 2020; 9(4): 170
[Pubmed] | [DOI]
113 Profiling of Chlorogenic Acids from Bidens pilosa and Differentiation of Closely Related Positional Isomers with the Aid of UHPLC-QTOF-MS/MS-Based In-Source Collision-Induced Dissociation
Anza-Tshilidzi Ramabulana, Paul Steenkamp, Ntakadzeni Madala, Ian A. Dubery
Metabolites. 2020; 10(5): 178
[Pubmed] | [DOI]
114 Biotechnological Interventions for Ginsenosides Production
Saikat Gantait, Monisha Mitra, Jen-Tsung Chen
Biomolecules. 2020; 10(4): 538
[Pubmed] | [DOI]
115 The Synergistic Effect of Co-Treatment of Methyl Jasmonate and Cyclodextrins on Pterocarpan Production in Sophora flavescens Cell Cultures
Soyoung Kim, Yu Jeong Jeong, Su Hyun Park, Sung-Chul Park, Saet Buyl Lee, Jiyoung Lee, Suk Weon Kim, Bo-Keun Ha, Hyun-Soon Kim, HyeRan Kim, Young Bae Ryu, Jae Cheol Jeong, Cha Young Kim
International Journal of Molecular Sciences. 2020; 21(11): 3944
[Pubmed] | [DOI]
116 Induction of Callogenesis, Organogenesis, and Embryogenesis in Non-Meristematic Explants of Bleeding Heart and Evaluation of Chemical Diversity of Key Metabolites from Callus
Dariusz Kulus, Alicja Tymoszuk
International Journal of Molecular Sciences. 2020; 21(16): 5826
[Pubmed] | [DOI]
117 In Vitro Propagation and Variation of Antioxidant Properties in Micropropagated Vaccinium Berry Plants—A Review
Samir C. Debnath, Juran C. Goyali
Molecules. 2020; 25(4): 788
[Pubmed] | [DOI]
118 Characterization of (2E,6E)-3,7,11-Trimethyldodeca-2,6,10-Trien-1-Ol with Antioxidant and Antimicrobial Potentials from Euclea Crispa (Thunb.) Leaves
Chella Perumal Palanisamy, Bo Cui, Hong Xia Zhang, Thanh Trung Nguyen, Hoang Dung Tran, Tran Dang Khanh, Van Quan Nguyen, Tran Dang Xuan
International Letters of Natural Sciences. 2020; 80: 51
[Pubmed] | [DOI]
119 Stimulation of 6-benzylaminopurine and meta-topolin-induced in vitro shoot organogenesis and production of flavonoids of Amburana cearensis (Allemăo) A.C. Smith)
Jéssica Nascimento Costa Vasconcelos, Alone Lima Brito, Amanda Lima Pinheiro, Dinah Ise Jimenez Gonçalves e Costa Pinto, Jackson Roberto Guedes da Silva Almeida, Taliane Leila Soares, José Raniere Ferreira de Santana
Biocatalysis and Agricultural Biotechnology. 2019; 22: 101408
[Pubmed] | [DOI]
120 Secondary Metabolism and Interspecific Competition Affect Accumulation of Spontaneous Mutants in the GacS-GacA Regulatory System in Pseudomonas protegens
Qing Yan, Lucas D. Lopes, Brenda T. Shaffer, Teresa A. Kidarsa, Oliver Vining, Benjamin Philmus, Chunxu Song, Virginia O. Stockwell, Jos M. Raaijmakers, Kerry L. McPhail, Fernando D. Andreote, Jeff H. Chang, Joyce E. Loper, Steven E. Lindow
mBio. 2018; 9(1)
[Pubmed] | [DOI]
121 Modularization and Response Curve Engineering of a Naringenin-Responsive Transcriptional Biosensor
Brecht De Paepe, Jo Maertens, Bartel Vanholme, Marjan De Mey
ACS Synthetic Biology. 2018; 7(5): 1303
[Pubmed] | [DOI]
122 A novel miRNA analysis framework to analyze differential biological networks
Ankush Bansal,Tiratha Raj Singh,Rajinder Singh Chauhan
Scientific Reports. 2017; 7(1)
[Pubmed] | [DOI]
123 Nanomaterials in plant tissue culture: the disclosed and undisclosed
Doo Hwan Kim,Judy Gopal,Iyyakkannu Sivanesan
RSC Adv.. 2017; 7(58): 36492
[Pubmed] | [DOI]
124 Chemometric evaluation of hypericin and related phytochemicals in 17 in vitro cultured Hypericum species, hairy root cultures and hairy root-derived transgenic plants
Katarína Nigutová,Souvik Kusari,Selahaddin Sezgin,Linda Petijová,Jana Henzelyová,Miroslava Bálintová,Michael Spiteller,Eva Cellárová
Journal of Pharmacy and Pharmacology. 2017;
[Pubmed] | [DOI]
125 Abiotic factors influencing podophyllotoxin and yatein overproduction in Leptohyptis macrostachys cultivated in vitro
Paloma R. Meira,Juceni P. David,Erika M. de O. Ribeiro,José R.F. Santana,Hugo N. Brandăo,Lenaldo M. de Oliveira,Jorge M. David,Héctor H. Medrado,José F.B. Pastore
Phytochemistry Letters. 2017; 22: 287
[Pubmed] | [DOI]
126 Development of botanicals to combat antibiotic resistance
Pooja D. Gupta,Tannaz J. Birdi
Journal of Ayurveda and Integrative Medicine. 2017;
[Pubmed] | [DOI]
127 Plant flavonoids in cancer chemoprevention: role in genome stability
Vazhappilly Cijo George,Graham Dellaire,H.P. Vasantha Rupasinghe
The Journal of Nutritional Biochemistry. 2017; 45: 1
[Pubmed] | [DOI]
128 Antimicrobial activity and acetylcholinesterase inhibition by extracts from chromatin modulated fungi
Matheus Thomaz Nogueira Silva Lima,Larissa Batista dos Santos,Rafael Wesley Bastos,Jacques Robert Nicoli,Jacqueline Aparecida Takahashi
Brazilian Journal of Microbiology. 2017;
[Pubmed] | [DOI]
129 Antioxidant and anticancer potential of bioactive compounds following UV-C light-induced plant cambium meristematic cell cultures
So Hyun Moon,Bhupendra Mistry,Doo Hwan Kim,Muthuraman Pandurangan
Industrial Crops and Products. 2017; 109: 762
[Pubmed] | [DOI]
130 Enhanced production of vanillin flavour metabolites by precursor feeding in cell suspension cultures of Decalepis hamiltonii Wight & Arn., in shake flask culture
Pradeep Matam,Giridhar Parvatam,Nandini P. Shetty
3 Biotech. 2017; 7(6)
[Pubmed] | [DOI]
131 Transcriptome analysis of Dioscorea zingiberensis identifies genes involved in diosgenin biosynthesis
Wenping Hua,Weiwei Kong,XiaoYan Cao,Chen Chen,Qian Liu,Xiangmin Li,Zhezhi Wang
Genes & Genomics. 2017;
[Pubmed] | [DOI]
132 Comprehensive assessment of the genes involved in withanolide biosynthesis from Withania somnifera: chemotype-specific and elicitor-responsive expression
Aditya Vikram Agarwal,Parul Gupta,Deeksha Singh,Yogeshwar Vikram Dhar,Deepak Chandra,Prabodh Kumar Trivedi
Functional & Integrative Genomics. 2017;
[Pubmed] | [DOI]
133 Phytochemistry and biological properties of Aristotelia chilensis a Chilean blackberry: a review
Gustavo E. Zúńiga,Andrea Tapia,Andrea Arenas,Rodrigo A. Contreras,Gustavo Zúńiga-Libano
Phytochemistry Reviews. 2017;
[Pubmed] | [DOI]
134 Biotechnological aspects of the production of natural sweetener glycyrrhizin from Glycyrrhiza sp.
Devendra Kumar Pandey,N. W. Ayangla
Phytochemistry Reviews. 2017;
[Pubmed] | [DOI]
135 Aluminum chloride elicitation (amendment) improves callus biomass growth and reserpine yield in Rauvolfia serpentina leaf callus
Nadia Zafar,A. Mujib,Muzamil Ali,Dipti Tonk,Basit Gulzar
Plant Cell, Tissue and Organ Culture (PCTOC). 2017;
[Pubmed] | [DOI]
136 Antioxidant and cytotoxic activity of bioactive phenolic metabolites isolated from the yeast-extract treated cell culture of apple
Amol Sarkate,Somesh Banerjee,Javid Iqbal Mir,Partha Roy,Debabrata Sircar
Plant Cell, Tissue and Organ Culture (PCTOC). 2017;
[Pubmed] | [DOI]
137 Secondary metabolism of pharmaceuticals in the plant in vitro cultures: strategies, approaches, and limitations to achieving higher yield
Tasiu Isah,Shahid Umar,Abdul Mujib,Maheshwar Prasad Sharma,P. E. Rajasekharan,Nadia Zafar,Arajmand Frukh
Plant Cell, Tissue and Organ Culture (PCTOC). 2017;
[Pubmed] | [DOI]
Mateusz Kawka, Maciej Pilarek, Katarzyna Syklowska-Baranek, Agnieszka Pietrosiuk
Prospects in Pharmaceutical Sciences. 2017; 15(7): 60
[Pubmed] | [DOI]
139 Production of Recombinant Anti-Cancer Vaccines in Plants
Jeong Hwan Lee,Kisung Ko
Biomolecules & Therapeutics. 2017; 25(4): 345
[Pubmed] | [DOI]
140 Exogenous Feeding of Fructose and Phenylalanine Further Improves Betulin Production in Suspended Betula platyphylla Cells under Nitric Oxide Treatment
Guizhi Fan,Tingting Nie,Jin Fan,Yaguang Zhan
Molecules. 2017; 22(7): 1035
[Pubmed] | [DOI]
141 Transcriptome Analysis of Secondary Metabolism Pathway, Transcription Factors, and Transporters in Response to Methyl Jasmonate in Lycoris aurea
Rong Wang,Sheng Xu,Ning Wang,Bing Xia,Yumei Jiang,Ren Wang
Frontiers in Plant Science. 2017; 7
[Pubmed] | [DOI]
142 Isolation and Identification of Streptomyces ramulosus from Soil and Determination of Antimicrobial Property of its Pigment
Saba Azimi, Majid Basei Salehi, Nima Bahador
Modern Medical Laboratory Journal. 2017; 1(1): 36
[Pubmed] | [DOI]
143 Expresión de la proteína recombinante Cry 1Ac en cultivos de células de papa en suspensión: Establecimiento del cultivo y optimización de la producción de la biomasa y la proteína mediante la adición de nitrógeno
Carlos Julio Nova-López,Jorge Mario Muńoz-Pérez,Luisa Fernanda Granger-Serrano,Mario Eveilio Arias-Zabala,Rafael Eduardo Arango-Isaza
DYNA. 2017; 84(201): 34
[Pubmed] | [DOI]
144 Effects of Abies sibirica terpenes on cancer- and aging-associated pathways in human cells
Anna Kudryavtseva, George Krasnov, Anastasiya Lipatova, Boris Alekseev, Faniya Maganova, Mikhail Shaposhnikov, Maria Fedorova, Anastasiya Snezhkina, Alexey Moskalev
Oncotarget. 2016; 7(50): 83744
[Pubmed] | [DOI]
145 Endophytes: A Treasure House of Bioactive Compounds of Medicinal Importance
Sushanto Gouda,Gitishree Das,Sandeep K. Sen,Han-Seung Shin,Jayanta Kumar Patra
Frontiers in Microbiology. 2016; 7
[Pubmed] | [DOI]
146 Chemical Elicitor-Induced Modulation of Antioxidant Metabolism and Enhancement of Secondary Metabolite Accumulation in Cell Suspension Cultures of Scrophularia kakudensis Franch
Abinaya Manivannan,Prabhakaran Soundararajan,Yoo Park,Byoung Jeong
International Journal of Molecular Sciences. 2016; 17(3): 399
[Pubmed] | [DOI]
147 Molecular Approaches to Genetically Improve the Accumulation of Health-Promoting Secondary Metabolites in Staple Crops—A Case Study: The Lipoxygenase-B1 Genes and Regulation of the Carotenoid Content in Pasta Products
Grazia Borrelli,Daniela Trono
International Journal of Molecular Sciences. 2016; 17(7): 1177
[Pubmed] | [DOI]
148 Enhanced camptothecin production induced by elicitors in the cell suspension cultures of Ophiorrhiza mungos Linn.
S. Deepthi,K. Satheeshkumar
Plant Cell, Tissue and Organ Culture (PCTOC). 2016; 124(3): 483
[Pubmed] | [DOI]
149 Chemical elicitors versus secondary metabolite production in vitro using plant cell, tissue and organ cultures: recent trends and a sky eye view appraisal
Charu Chandra Giri,Mohd Zaheer
Plant Cell, Tissue and Organ Culture (PCTOC). 2016;
[Pubmed] | [DOI]
150 Enhanced plumbagin production in Plumbago indica root cultures by ?-alanine feeding and in situ adsorption
Amit Jaisi,Pharkphoom Panichayupakaranant
Plant Cell, Tissue and Organ Culture (PCTOC). 2016;
[Pubmed] | [DOI]
151 Cell line selection combined with jasmonic acid elicitation enhance camptothecin production in cell suspension cultures of Ophiorrhiza mungos L
S. Deepthi,K. Satheeshkumar
Applied Microbiology and Biotechnology. 2016;
[Pubmed] | [DOI]
152 An optimized biotechnological system for the production of centellosides based on elicitation and bioconversion of Centella asiatica cell cultures
Diego Hidalgo,Virginie Steinmetz,Maude Brossat,Lucie Tournier-Couturier,Rosa M. Cusido,Purificacion Corchete,Javier Palazon
Engineering in Life Sciences. 2016;
[Pubmed] | [DOI]
153 Statistical experimental designs for the production of secondary metabolites in plant cell suspension cultures
Christian Schmitz,Leonie Fritsch,Rainer Fischer,Stefan Schillberg,Stefan Rasche
Biotechnology Letters. 2016;
[Pubmed] | [DOI]
154 Increasing the synthesis of bioactive abietane diterpenes in Salvia sclarea hairy roots by elicited transcriptional reprogramming
M. C. Vaccaro,A. Mariaevelina,N. Malafronte,N. De Tommasi,A. Leone
Plant Cell Reports. 2016;
[Pubmed] | [DOI]
155 Modulation of Picroside-I Biosynthesis in Grown Elicited Shoots of Picrorhiza kurroa In Vitro
Neha Sharma,Varun Kumar,Rajinder Singh Chauhan,Hemant Sood
Journal of Plant Growth Regulation. 2016;
[Pubmed] | [DOI]
156 Micropropagation and callus induction of Lantana camara L. - a medicinal plant
Varaporn Veraplakorn
Agriculture and Natural Resources. 2016;
[Pubmed] | [DOI]
157 Production of camptothecin in the elicited callus cultures of Nothapodytes nimmoniana (J. Graham) Mabberly
Tasiu Isah
Chemical Papers. 2016;
[Pubmed] | [DOI]
158 Transformation of Lactuca sativa L. with rol C gene results in increased antioxidant potential and enhanced analgesic, anti-inflammatory and antidepressant activities in vivo
Hammad Ismail,Erum Dilshad,Mohammad Tahir Waheed,Moniba Sajid,Waqas Khan Kayani,Bushra Mirza
3 Biotech. 2016; 6(2)
[Pubmed] | [DOI]
159 Can Ocimum basilicum L. and Ocimum tenuiflorum L. in vitro culture be a potential source of secondary metabolites?
Karuppiah Bhuvaneshwari,Ananda Gokulanathan,Malayandi Jayanthi,Vaithiyanathan Govindasamy,Luigi Milella,Sungyoung Lee,Deok Chun Yang,Shanmugam Girija
Food Chemistry. 2016; 194: 55
[Pubmed] | [DOI]
160 Production of rosmarinic acid and salvianolic acid B from callus culture of Salvia miltiorrhiza with cytotoxicity towards acute lymphoblastic leukemia cells
Ching-Fen Wu,Anastasia Karioti,Doris Rohr,Anna Rita Bilia,Thomas Efferth
Food Chemistry. 2016; 201: 292
[Pubmed] | [DOI]
161 Bioactive compounds in hyperhydric and normal micropropagated shoots of Aronia melanocarpa (Michx.) Elliott
Iyyakkannu Sivanesan,Ramesh Kumar Saini,Doo Hwan Kim
Industrial Crops and Products. 2016; 83: 31
[Pubmed] | [DOI]
162 Induction of aromatic amino acids and phenylpropanoid compounds in Scrophularia striata Boiss. cell culture in response to chitosan-induced oxidative stress
Maryam Kamalipourazad,Mohsen Sharifi,Hassan Zare Maivan,Mehrdad Behmanesh,Najmeh Ahmadian Chashmi
Plant Physiology and Biochemistry. 2016; 107: 374
[Pubmed] | [DOI]
163 Biotechnological interventions for harnessing podophyllotoxin from plant and fungal species: current status, challenges, and opportunities for its commercialization
Anita Kumari,Dharam Singh,Sanjay Kumar
Critical Reviews in Biotechnology. 2016; : 1
[Pubmed] | [DOI]
164 Plant Omics: Isolation, Identification, and Expression Analysis of Cytochrome P450 Gene Sequences fromColeus forskohlii
Praveen Awasthi,Vidushi Mahajan,Irshad Ahmad Rather,Ajai Prakash Gupta,Shafaq Rasool,Yashbir S. Bedi,Ram A. Vishwakarma,Sumit G. Gandhi
OMICS: A Journal of Integrative Biology. 2015; 19(12): 782
[Pubmed] | [DOI]
165 Assessment of the antiproliferative activity on murine melanoma cells of extracts from elicited cell suspensions of strawberry, strawberry tree, blackberry and red raspberry
C. Forni,A. Frattarelli,A. Lentini,S. Beninati,S. Lucioli,E. Caboni
Plant Biosystems - An International Journal Dealing with all Aspects of Plant Biology. 2015; : 1
[Pubmed] | [DOI]
166 The content of free and esterified triterpenoids of the native marigold (Calendula officinalis) plant and its modifications in in vitro cultures
Bartosz Nizynski,Abdulwadood Shakir Mahmood Alsoufi,Cezary Paczkowski,Marek Dlugosz,Anna Szakiel
Phytochemistry Letters. 2015; 11: 410
[Pubmed] | [DOI]
167 Anti-inflammatory and antioxidative activity of anthocyanins from purple basil leaves induced by selected abiotic elicitors
Urszula Szymanowska,Urszula Zlotek,Monika Karas,Barbara Baraniak
Food Chemistry. 2015; 172: 71
[Pubmed] | [DOI]
168 Biotechnological advances in UDP-sugar based glycosylation of small molecules
Frederik De Bruyn,Jo Maertens,Joeri Beauprez,Wim Soetaert,Marjan De Mey
Biotechnology Advances. 2015; 33(2): 288
[Pubmed] | [DOI]
169 Characterisation of the membrane transport of pilocarpine in cell suspension cultures of Pilocarpus microphyllus
Nathalia Luiza Andreazza,Ilka Nacif Abreu,Alexandra Christine Helena Franklan Sawaya,Paulo Mazzafera
Journal of Plant Physiology. 2015; 175: 37
[Pubmed] | [DOI]
170 A split airlift bioreactor for continuous culture of Glycyrrhiza inflata cell suspensions
G. R. Wang,Q. Z. Chen,N. Tang,B. L. Li,D. L. Fan,K. X. Tang
Plant Cell, Tissue and Organ Culture (PCTOC). 2015; 121(1): 121
[Pubmed] | [DOI]
171 Next-generation sequencing (NGS) transcriptomes reveal association of multiple genes and pathways contributing to secondary metabolites accumulation in tuberous roots of Aconitum heterophyllum Wall.
Tarun Pal,Nikhil Malhotra,Sree Krishna Chanumolu,Rajinder Singh Chauhan
Planta. 2015; 242(1): 239
[Pubmed] | [DOI]
172 Enhanced disease resistance in the Indian snakehead, Channa punctata against Aeromonas hydrophila, through 5 % feed supplementation with F. benghalensis (aerial root) and L. leucocephala (pod seed)
Vipin Kumar Verma,Kumari Vandana Rani,Neeta Sehgal,Om Prakash
Aquaculture International. 2015; 23(5): 1127
[Pubmed] | [DOI]
173 Influence of Agrobacterium oncogenes on secondary metabolism of plants
Tatiana V. Matveeva,Sophie V. Sokornova,Ludmila A. Lutova
Phytochemistry Reviews. 2015; 14(3): 541
[Pubmed] | [DOI]
174 In Vitro Cultivars of Vaccinium corymbosum L. (Ericaceae) are a Source of Antioxidant Phenolics
Rodrigo Contreras,Hans Köhler,Marisol Pizarro,Gustavo Zúiga
Antioxidants. 2015; 4(2): 281
[Pubmed] | [DOI]
175 Selection and validation of reference genes for quantitative real-time PCR analysis of gene expression in Cichorium intybus
Marianne Delporte,Guillaume Legrand,Jean-Louis Hilbert,David Gagneul
Frontiers in Plant Science. 2015; 6
[Pubmed] | [DOI]
176 Metabolic engineering of Escherichia coli into a versatile glycosylation platform: production of bio-active quercetin glycosides
Frederik De Bruyn,Maarten Van Brempt,Jo Maertens,Wouter Van Bellegem,Dries Duchi,Marjan De Mey
Microbial Cell Factories. 2015; 14(1)
[Pubmed] | [DOI]
177 In Vitro Production of Echioidinin, 7-O-Methywogonin from Callus Cultures of Andrographis lineata and Their Cytotoxicity on Cancer Cells
Arifullah Mohammed,Kishore K. Chiruvella,Yerra Koteswara Rao,Madamanchi Geethangili,Sathees C. Raghavan,Rama Gopal Ghanta,Brett Neilan
PLOS ONE. 2015; 10(10): e0141154
[Pubmed] | [DOI]
178 Cell cultures of Maytenus ilicifolia Mart. are richer sources of quinone-methide triterpenoids than plant roots in natura
Juliana S. Coppede,Edieidia S. Pina,Tiago A. Paz,Ana L. Fachin,Mozart A. Marins,Bianca W. Bertoni,Suzelei C. França,Ana Maria S. Pereira
Plant Cell, Tissue and Organ Culture (PCTOC). 2014;
[Pubmed] | [DOI]
179 Oxidative stress and production of bioactive monoterpene indole alkaloids: biotechnological implications
Hélio Nitta Matsuura,Mariana Ritter Rau,Arthur Germano Fett-Neto
Biotechnology Letters. 2014; 36(2): 191-200
[Pubmed] | [DOI]
180 New trends in biotechnological production of rosmarinic acid
Abbas Khojasteh,Mohammad Hossein Mirjalili,Diego Hidalgo,Purificación Corchete,Javier Palazon
Biotechnology Letters. 2014;
[Pubmed] | [DOI]
181 Effect of Salicylic Acid on the Activity of PAL and PHB Geranyltransferase and Shikonin Derivatives Production in Cell Suspension Cultures of Arnebia euchroma (Royle) Johnst—a Medicinally Important Plant Species
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Applied Biochemistry and Biotechnology. 2014;
[Pubmed] | [DOI]
182 Prospects for the use of plant cell cultures in food biotechnology
Kevin M Davies,Simon C Deroles
Current Opinion in Biotechnology. 2014; 26: 133
[Pubmed] | [DOI]
183 Biotechnological production of natural zero-calorie sweeteners
Ryan N Philippe,Marjan De Mey,Jeff Anderson,Parayil Kumaran Ajikumar
Current Opinion in Biotechnology. 2014; 26: 155
[Pubmed] | [DOI]
184 Callus culture development of two varieties of Tagetes erecta and carotenoid production
Israel Benítez-García,Pablo Emilio Vanegas-Espinoza,Antonio J. Meléndez-Martínez,Francisco J. Heredia,Octavio Paredes-López,Alma Angélica Del Villar-Martínez
Electronic Journal of Biotechnology. 2014;
[Pubmed] | [DOI]
185 Isolation of pomolic acid from Chamaenerion angustifolium and the evaluation of its potential genotoxicity in bacterial test systems
T. S. Frolova,O. I. Sal’nikova,T. A. Dudareva,T. P. Kukina,O. I. Sinitsyna
Russian Journal of Bioorganic Chemistry. 2014; 40(1): 82
[Pubmed] | [DOI]
186 Isoflavone Augmentation in Soybean Cell Cultures Is Optimized Using Response Surface Methodology
M. K. Akitha Devi,P. Giridhar
Journal of Agricultural and Food Chemistry. 2014; 62(14): 3143
[Pubmed] | [DOI]
187 Isolation and characterization of diosgenin from in vitro cultured tissues of Helicteres isora L
Harshal A. Deshpande,Sanjivani R. Bhalsing
Physiology and Molecular Biology of Plants. 2013;
[Pubmed] | [DOI]
188 Enhanced production of napthoquinone metabolite (shikonin) from cell suspension culture of Arnebia sp. and its up-scaling through bioreactor
Komal Gupta,Shashank Garg,Joginder Singh,Manoj Kumar
3 Biotech. 2013;
[Pubmed] | [DOI]
189 Thidiazuron-Induced Changes in Biomass Parameters, Total Phenolic Content, and Antioxidant Activity in Callus Cultures of Artemisia absinthium L.
Mohammad Ali,Bilal Haider Abbasi
Applied Biochemistry and Biotechnology. 2013;
[Pubmed] | [DOI]
190 Current approaches for enhanced expression of secondary metabolites as bioactive compounds in plants for agronomic and human health purposes - A review
Jimenez-Garcia, S.N., Vazquez-Cruz, M.A., Guevara-Gonzalez, R.G., Torres-Pacheco, I., Cruz-Hernandez, A., Feregrino-Perez, A.A.
Polish Journal of Food and Nutrition Sciences. 2013; 63(2): 67-78
191 Advances of paclitaxel combinatorial biosynthesis in yeast
Wang, Y.-X. and Wang, C.-M.
Chinese Journal of Pharmaceutical Biotechnology. 2013; 20(3): 271-275
192 Salicylic acid elicitation on production of secondary metabolite by cell cultures of Jatropha Curcas L
Mahalakshmi, R., Eganathan, P., Parida, A.K.
International Journal of Pharmacy and Pharmaceutical Sciences. 2013; 5(4): 655-659
193 Effects of zinc and cadmium ions on cell growth and production of coumarins in cell suspension cultures of Angelica archangelica L.
Siatka, T., Kašparová, M.
Ceska a Slovenska Farmacie. 2012; 61(6): 261-266
194 Proteomic profiling of the 11-dehydrosinulariolide-treated oral carcinoma cells Ca9-22: Effects on the cell apoptosis through mitochondrial-related and ER stress pathway
Liu, C.-I. and Wang, R.Y.L. and Lin, J.-J. and Su, J.-H. and Chiu, C.-C. and Chen, J.-C. and Chen, J.Y.F. and Wu, Y.-J.
Journal of Proteomics. 2012; 75(18): 5578-5589
195 Proteomic profiling of the 11-dehydrosinulariolide-treated oral carcinoma cells Ca9–22: Effects on the cell apoptosis through mitochondrial-related and ER stress pathway
Chih-I Liu,Robert Yung-Liang Wang,Jen-Jie Lin,Jui-Hsin Su,Chien-Chih Chiu,Jiing-Chuan Chen,Jeff Yi-Fu Chen,Yu-Jen Wu
Journal of Proteomics. 2012; 75(18): 5578
[Pubmed] | [DOI]
196 Plant Biotechnology for Industrial Production
Shinsaku TAKAYAMA,Nobuaki MERA,Motomu AKITA
Shokubutsu Kankyo Kogaku. 2012; 24(4): 224
[Pubmed] | [DOI]


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