| ORIGINAL ARTICLE |
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| Year : 2012 | Volume
: 4
| Issue : 4 | Page : 318-321 |
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Determination of azathioprine in bulk and pharmaceutical dosage form by HPTLC
Pritam S Jain, Pradip Thakre, Amar J Chaudhari, Manish L Chavhan, Sanjay J Surana
Department of Pharmaceutical Chemistry, R. C. Patel Institute of Pharmaceutical Education and Research, Shirpur, Dist. Dhule, India
Correspondence Address:
Pritam S Jain
Department of Pharmaceutical Chemistry, R. C. Patel Institute of Pharmaceutical Education and Research, Shirpur, Dist. Dhule,
India
 Source of Support: None, Conflict of Interest: None  | Check |
DOI: 10.4103/0975-7406.103263
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Introduction: An HPTLC method for analysis of Azathioprine in bulk and pharmaceutical formulation has been established and validated. Materials and Methods: The analyte was separated on aluminium plates precoated with silica gel 60 F254. The mobile phase was Ethyl acetate: Methanol: Triethylamine (4:1:0.5v/v). Quantification was done by densitometric scanning at 300nm. Results: Response was a linear function of Azathioprine concentration in the range of 200-1200 ng/band. The limit of detection and quantification for Azathioprine were 18.58 and 59.14 ng/band, respectively. Average recovery was 100.1% which shows that the method was free from interference from excipients present in the formulation. Conclusion: The established method enabled accurate, precise, and rapid analysis of Azathioprine in bulk as well as pharmaceutical formulation. |
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