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Year : 2015  |  Volume : 7  |  Issue : 4  |  Page : 260-263

A high performance thin layer chromatographic method for the estimation of colchicine in different formulations

1 Nandini Nagar College of Pharmacy, Nawabganj, Gonda, Uttar Pradesh, India
2 Department of Pharmacognosy and Phytochemistry, Faculty of Pharmacy, Bioactive Natural Product Laboratory, Hamdard University, New Delhi, India
3 SBS College of Pharmacy, Patti, Punjab, India

Correspondence Address:
Sayeed Ahmad
Department of Pharmacognosy and Phytochemistry, Faculty of Pharmacy, Bioactive Natural Product Laboratory, Hamdard University, New Delhi
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Source of Support: None, Conflict of Interest: None

DOI: 10.4103/0975-7406.168021

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Background: Colchicine is a main alkaloid present in bitter and sweet variety of colchicum (Colchicum luteum Baker), which have been reported to possess anti-rheumatic, anti-gout, and anticancer potential. Colchicum is an important ingredient of several Unani and Herbal formulations. Quantification of colchicine will play a great role in quality control of these formulations. Hence, a high-performance thin layer chromatographic (TLC) method has been developed for the analysis of colchicine in Unani formulations of various dosage forms such as hubb (tablet) and capsules. Materials and Methods: The samples were applied on aluminum TLC plates precoated with silica gel 60-F254and developed using mobile phase toluene-dichloromethane-methanol in equal proportions. Quantification was done by densitometric scanning at 350 nm, which showed a linear response in the range of 50–500 ng/spot. The developed method was validated as per the International Conference on Harmonization guidelines for linearity, precision, accuracy, specificity, robustness, limit of detection, and limit of quantification. Results and Conclusion: The developed method was applied for quantitative estimation of colchicine in different Unani and Herbal formulations. The method was found simple, selective, accurate with a wide range of linearity, hence suitable for the quality control of different formulations and varieties of colchicum with respect to colchicine content.

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