Evaluation of the antidiabetic potential of an isolated hydroalcoholic fraction from the fruit of withania coagulans
Md Waris1, Naiyer Shahzad2, Saeed Saeed Al-Ghamdi2, Showkat Rasool Mir3, Tanuja1
1 Department of Botany, Thakur Prasad Singh (TPS) College, Patna, Magadh University, Bodh Gaya; Department of Botany, Thakur Prasad Singh (TPS) College, Patliputra University, Patna, Bihar, India
2 Department of Pharmacology and Toxicology, Faculty of Medicine, Umm Al-Qura University, Makkah, Saudi Arabia
3 Department of Pharmacognosy and Phytochemistry, Phytopharmaceutical Research Laboratory, Faculty of Pharmacy, Jamia Hamdard, New Delhi, India
Department of Botany, Thakur Prasad Singh (TPS) College, Patliputra University, Patna - 800 001, Bihar
Source of Support: None, Conflict of Interest: None
The hydro-alcoholic extract of Withania coagulans fruits was investigated for preliminary phytochemical screening and characterized by high-performance thin-layer chromatography. Column chromatography of the hydro-alcoholic extract of W. coagulans eluted with four different combinations of ethyl acetate and methanol yielded four fractions (WCF01, WCF02, WCF03, and WCF04). One of these fractions, WCF02, significantly (P < 0.05) inhibited in vitro α-amylase and α-glucosidase activity with IC50 values of 104.71 μg/mL and 70.79 μg/mL, respectively. WCF02 further reduced blood glucose levels in comparison to control in the starch tolerance test. The extract showed a relative dose-dependent effect. It was observed that none of the extracts could delay the peak blood glucose that was achieved after 60 min of carbohydrate challenge, but these blunted the glycemic peak.