In Silico and In Vitro Investigation of Anti Helicobacter Activity of Selected Phytochemicals

Deniz Al Tawalbeh; Talal Aburjai; Qosay Al Balas; Ali Al Samydai

doi:10.4103/jpbs.jpbs_850_21

Journal of Pharmacy And Bioallied Sciences

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ORIGINAL ARTICLE

Year : 2022 | Volume : 14 | Issue : 3 | Page : 132-139

In Silico and In Vitro Investigation of Anti Helicobacter Activity of Selected Phytochemicals

Deniz Al Tawalbeh¹, Talal Aburjai², Qosay Al Balas³, Ali Al Samydai⁴
¹ Department of Medicinal Chemistry and Pharmacognosy, Faculty of Pharmacy, Yarmouk University, Irbid, Jordan
² Department of Pharmaceutical Sciences, Faculty of Pharmacy, University of Jordan, Amman, Jordan
³ Department of Medicinal Chemistry and Pharmacognosy, Faculty of Pharmacy, JUST University, Irbid, Jordan; Department Pharmacological, Diagnostic Research Centre, Faculty of Pharmacy, Al Ahliyya Amman University, Jordan
⁴ Department Pharmacological, Diagnostic Research Centre, Faculty of Pharmacy, Al Ahliyya Amman University, Jordan

Correspondence Address:
Dr. Talal Aburjai
Department of Pharmaceutical Sciences, Faculty of Pharmacy, University of Jordan, Amman
Jordan

Source of Support: None, Conflict of Interest: None

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DOI: 10.4103/jpbs.jpbs_850_21

Introduction: Helicobacter pylori is Gram-negative helical bacteria that inhibit stomach mucosal lining and establish infection. Urease enzyme was confirmed to be pivotal target in which its suppression will prompt bacteria treatment and eradication. Methods: Series of naturally bioactive compounds were selected based on ethnobotanical and molecular modeling techniques with potential urease inhibitory effect. The selected phytochemical compounds were in-silico and in-vitro assayed against urease enzyme, minimal inhibitory concentrations (MIC) and a synergistic effect was studied and cultured specifically for H. pylori. Results: Terpineol was considered as the most active compound with an IC₅₀ of 1.443 μg/ml (R² = 0.9374). The synergistic effect of terpineol and metronidazole indicated a possible additive effect (fractional inhibitory concentration result is 0.78) with improvement of MIC results for both terpineol and metronidazole. Conclusion: This study suggests that terpineol is best to be considered as a lead compound for H. pylori infection treatment and could be a potent inhibitor when combined with metronidazole targeting urease enzyme.

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