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Fresh juice and extract of Drynaria quercifolia rhizome nutraceutical for the management of arthritis with special emphasis on enzyme levels
Unnikrishnan Meenakshi Dhanalekshmi1, Thirulingam Gowri2, Balasundaram Ramya2, Rajagopal Srinivasan2
1 Organic and Bio Organic Chemistry Division, Central Leather Research Institute (Council of Scientific and Industrial Research), Chennai, Tamil Nadu, India; Department of Pharmacology, College of Pharmacy, National University of Science and Technology, Muscat, Sultanate of Oman
2 Organic and Bio Organic Chemistry Division, Central Leather Research Institute (Council of Scientific and Industrial Research), Chennai, Tamil Nadu, India
Correspondence Address:
Rajagopal Srinivasan,
Organic and Bio Organic Chemistry Division, Central Leather Research Institute (Council of Scientific and Industrial Research), Chennai 600020, Tamil Nadu.
India
 Source of Support: None, Conflict of Interest: None DOI: 10.4103/jpbs.JPBS_227_20
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Objective: The objective of this study was to screen fresh and concentrated juice extract of rhizome of Drynaria quercifolia (L.) for its anti-arthritic property using in vivo model. Materials and Methods: In vivo anti-arthritic effect was evaluated by complete Freund’s adjuvant (CFA)-induced arthritic model. Hematological and biochemical parameters were evaluated in the arthritic model with and without treatment. Assays of enzymatic and nonenzymatic antioxidants, lysosomal enzymes, and protein-bound carbohydrates were performed using the standard protocol. Histopathological examinations were carried out in different organ tissues. Results: Decrease in paw volume was noticed from 16th day and a significant reduction in paw volume was observed after 16th day in Drynaria Quercifolia Rhizome (DQR) (extract and fresh juice)-treated and methotrexate (MTx)-treated groups. Levels of hemoglobin, PCV (packed cell volume), and platelets were normal in all groups. Significant (P < 0.05) increase of TC (total count) and decrease of red blood cells were observed in the arthritic group (negative control) when compared with normal control. There was a significant change in liver enzyme levels. The activities of enzymatic antioxidants such as catalase, dismutase, and peroxidase in tissue homogenates also showed positive results. There was a significant (P < 0.05) increase in levels of lysosomal enzymes (alkaline phosphatase, acid phosphatase, and cathepsin D) in the arthritic control animals. These enzymes levels are being able to control by the treatment with DQR (extract and fresh juice) and standard drug (MTx). Histology reports confirm the presence of only a mild cartilage erosion in DQ fresh juice-treated group. Conclusion: Our findings pointed out the promising effect of fresh juice and extract of D. quercifolia Rhizome for the management of arthritis. |
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