Journal of Pharmacy And Bioallied Sciences

SHORT COMMUNICATION
Year
: 2010  |  Volume : 2  |  Issue : 4  |  Page : 369--371

Fluorescent labeling of human albumin using the new aromatic dialdehyde labels and the study of innerfilter effect


Muhammad Aminuddin1, Sofia Ahmed1, Muhammd Ali Sheraz1, Iqbal Ahmad1, Karamat Mahmood2, JN Miller3 
1 Institute of Pharmaceutical Sciences, Baqai Medical University, Toll Plaza, Super Highway, Gadap Road, Karachi - 746 00, Pakistan
2 Department of Chemistry, The Islamia University, Bahawalpur, Pakistan
3 Department of Analytical Chemistry, Loughborough University of Engineering & Technology, Loughborough, LE11, England

Correspondence Address:
Muhammd Ali Sheraz
Institute of Pharmaceutical Sciences, Baqai Medical University, Toll Plaza, Super Highway, Gadap Road, Karachi - 746 00
Pakistan

The labels naphthalene-2,3-dicarboxaldehyde (NDA), 1-phenylnaphthalene-2,3-dialdehyde (fNDA), and anthracene-2,3-dialdehyde (ADA) have been used as fluorigenic reagents. They formed fluorescent derivatives with proteins. The derivatives formed are in fact isoindoles. The fluorescence decay of the labels-antibody was found to extend over a period of 4, 8, and 10 h for ΦNDA, ADA, and NDA-derivative, respectively. Protein formed is comparatively less stable as compared to simple amino acids. In relation to innerfilter effect, the addition of cytochrome C, myoglobin, and ATP as absorbers to label-human albumin fluorophores appeared to have quenched the fluorescence. In the case of using NDA as label, the fluorescence was quenched roughly 70%, 24%, and 58% for addition of cytochrome C, myoglobin, and ATP, respectively. The labels used were found to give rapid, reproducible, and reliable results.


How to cite this article:
Aminuddin M, Ahmed S, Sheraz MA, Ahmad I, Mahmood K, Miller J N. Fluorescent labeling of human albumin using the new aromatic dialdehyde labels and the study of innerfilter effect.J Pharm Bioall Sci 2010;2:369-371


How to cite this URL:
Aminuddin M, Ahmed S, Sheraz MA, Ahmad I, Mahmood K, Miller J N. Fluorescent labeling of human albumin using the new aromatic dialdehyde labels and the study of innerfilter effect. J Pharm Bioall Sci [serial online] 2010 [cited 2022 Jun 26 ];2:369-371
Available from: https://www.jpbsonline.org/article.asp?issn=0975-7406;year=2010;volume=2;issue=4;spage=369;epage=371;aulast=Aminuddin;type=0