Journal of Pharmacy And Bioallied Sciences

RESEARCH ARTICLE
Year
: 2012  |  Volume : 4  |  Issue : 4  |  Page : 276--281

Simultaneous quantification of stevioside and rebaudioside A in different stevia samples collected from the Indian subcontinent


Karishma Chester, Ennus T Tamboli, Mhaveer Singh, Sayeed Ahmad 
 Department of Pharmacognosy and Phytochemistry,Faculty of Pharmacy,Jamia Hamdard, New Delhi, India

Correspondence Address:
Sayeed Ahmad
Department of Pharmacognosy and Phytochemistry,Faculty of Pharmacy,Jamia Hamdard, New Delhi
India

Background: A high performance thin layer chromatographic (HPTLC) method was developed for simultaneous estimation of stevioside and rebaudioside A in Stevia rebaudiana samples collected from different regions of Indian subcontinent. Materials and Methods: The separation was achieved by using acetone: ethyl acetate: water (5:4:1, v/v/v) as the solvent system on precoated silica gel 60 F 254 TLC plates. The densitometric quantification of stevia glycosides was carried out at wavelength 360 nm in absorption mode after spraying with anisaldehyde sulphuric acid as detecting reagent. Results: The well resolved peaks for stevioside and rebaudioside A were observed at R f values 0.31± 0.02 and 0.21± 0.02 respectively. The calibration curves were found linear with a wide range of concentration 100 - 2000 ng spot -1 with good correlation coefficient 0.996 and 0.991 for stevioside and rebaudioside A, respectively. Conclusions: The proposed method was validated as per the ICH (International Conferences on Harmonization) guidelines and found simple, sensitive, economic, reproducible, robust and accurate for quantitative analysis of stevia glycosides, which can be applied for quality control of stevia as well as to check.


How to cite this article:
Chester K, Tamboli ET, Singh M, Ahmad S. Simultaneous quantification of stevioside and rebaudioside A in different stevia samples collected from the Indian subcontinent.J Pharm Bioall Sci 2012;4:276-281


How to cite this URL:
Chester K, Tamboli ET, Singh M, Ahmad S. Simultaneous quantification of stevioside and rebaudioside A in different stevia samples collected from the Indian subcontinent. J Pharm Bioall Sci [serial online] 2012 [cited 2022 Aug 8 ];4:276-281
Available from: https://www.jpbsonline.org/article.asp?issn=0975-7406;year=2012;volume=4;issue=4;spage=276;epage=281;aulast=Chester;type=0