Journal of Pharmacy And Bioallied Sciences

ORIGINAL ARTICLE
Year
: 2021  |  Volume : 13  |  Issue : 4  |  Page : 367--372

Evaluation of the antidiabetic potential of an isolated hydroalcoholic fraction from the fruit of withania coagulans


Md Waris1, Naiyer Shahzad2, Saeed Saeed Al-Ghamdi2, Showkat Rasool Mir3, Tanuja1 
1 Department of Botany, Thakur Prasad Singh (TPS) College, Patna, Magadh University, Bodh Gaya; Department of Botany, Thakur Prasad Singh (TPS) College, Patliputra University, Patna, Bihar, India
2 Department of Pharmacology and Toxicology, Faculty of Medicine, Umm Al-Qura University, Makkah, Saudi Arabia
3 Department of Pharmacognosy and Phytochemistry, Phytopharmaceutical Research Laboratory, Faculty of Pharmacy, Jamia Hamdard, New Delhi, India

Correspondence Address:
Dr. Tanuja
Department of Botany, Thakur Prasad Singh (TPS) College, Patliputra University, Patna - 800 001, Bihar
India

The hydro-alcoholic extract of Withania coagulans fruits was investigated for preliminary phytochemical screening and characterized by high-performance thin-layer chromatography. Column chromatography of the hydro-alcoholic extract of W. coagulans eluted with four different combinations of ethyl acetate and methanol yielded four fractions (WCF01, WCF02, WCF03, and WCF04). One of these fractions, WCF02, significantly (P < 0.05) inhibited in vitro α-amylase and α-glucosidase activity with IC50 values of 104.71 μg/mL and 70.79 μg/mL, respectively. WCF02 further reduced blood glucose levels in comparison to control in the starch tolerance test. The extract showed a relative dose-dependent effect. It was observed that none of the extracts could delay the peak blood glucose that was achieved after 60 min of carbohydrate challenge, but these blunted the glycemic peak.


How to cite this article:
Waris M, Shahzad N, Al-Ghamdi SS, Mir SR, Tanuja. Evaluation of the antidiabetic potential of an isolated hydroalcoholic fraction from the fruit of withania coagulans.J Pharm Bioall Sci 2021;13:367-372


How to cite this URL:
Waris M, Shahzad N, Al-Ghamdi SS, Mir SR, Tanuja. Evaluation of the antidiabetic potential of an isolated hydroalcoholic fraction from the fruit of withania coagulans. J Pharm Bioall Sci [serial online] 2021 [cited 2022 May 25 ];13:367-372
Available from: https://www.jpbsonline.org/article.asp?issn=0975-7406;year=2021;volume=13;issue=4;spage=367;epage=372;aulast=Waris;type=0