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  Indian J Med Microbiol
 

Figure 1: Tm plot of lysozyme at 200 mg/mL (a) Plot of second derivative curve intensity of lysozyme at 1654/cm-1 against temperature (25-95°C) at pH 5.0. The inset is a similar plot where Tm is calculated from the sigmoid fitting between 50°C and 95°C at pH 5.0. (b) Bar diagram of Tm plot of lysozyme at varying pH calculated between 50°C and 95°C. For the transition temperature values [Table 2]

Figure 1: T<sub>m</sub> plot of lysozyme at 200 mg/mL (a) Plot of second derivative curve intensity of lysozyme at 1654/cm-1 against temperature (25-95°C) at pH 5.0. The inset is a similar plot where T<sub>m</sub> is calculated from the sigmoid fitting between 50°C and 95°C at pH 5.0. (b) Bar diagram of T<sub>m</sub> plot of lysozyme at varying pH calculated between 50°C and 95°C. For the transition temperature values [Table 2]